The Power of Precision
ZenoTOF 7600 system
TripleTOF 6600+ system
SCIEX 7500 system
SCIEX Triple Quad 6500+ system
SCIEX 5500+ system
QTRAP 6500+ system
QTRAP 4500 system
View all mass
Echo MS system
2.0 High Throughput Multiplexing system
View all integrated
BioPhase 8800 system
8000 Plus system
800 Plus system
Advance your research with front-end
instruments designed to help you realize the full power of your mass
spectrometer. SCIEX has the broadest portfolio of ESI-MS front-ends
can facilitate various flow rates, sample requirements and
sensitivities. No one else offers the entire range
flow, microflow, nanoflow LC-MS and even ultra-low flow
ExionLC 2.0 series
View all front-end HPLC MS
Differential mobility spectrometry (DMS) and
ion mobility spectrometry are analytical techniques used to separate
ions based on their gas phase mobility. Multiple types of ion
devices exist, such as drift tubes, traveling wave, and high-field
asymmetric waveform devices. Learn how you can separate yourself
Differential Ion Mobility.
The latest ion sources from SCIEX enable
enhanced sensitivity and robustness with greater desolvation range
across all MS platforms, from Triple Quad to QTRAP and QTOF.
Turbo V ion
OptiFlow Turbo V ion
View all ion sources
vMethod applications are pre-configured and verified LC-MS/MS
that reduce the need for method development – significantly cutting
time, effort and money to deploy a new assay. Every vMethod provides
method conditions, recommended sample prep, LC and MS conditions,
details for applicable MS/MS library databases for key applications.
and personal care products (PPCPs)
and protein bioanalysis
Explore vMethod applications
Free software trials
feature request portal
Analyst TF software
Biologics Explorer software
Molecule Profiler software
High resolution and QTRAP
libraries can dramatically enhance the quality of your analysis,
you much improved confidence in your data. With a comprehensive
at your fingertips, you can easily create methods and process
and non-targeted screening data on your complex samples, faster and
easier than ever before.
All in one
HR-MS/MS library with NIST 2017
Explore the library
Boost the performance of your mass
spectrometer and improve sensitivity, productivity, and data
iChemistry Solutions are the world's only reagents and
that are custom designed with your success in mind.
RNA 9000 Purity & Integrity kit
kit for amino acid analysis of hydrolysates
kit for amino acid analysis of physiological fluids
protein digestion solution
Protein CE-SDS Purity Analysis kit
protein assay - human induction kit
CZE rapid charge variant analysis kit
Fast Glycan Analysis and Labeling kit
standards kit for pesticide analysis
auEChERS extraction and dispersive kit
Ampliflex keto reagent
Cleavable ICAT reagent
View all consumables
QTOF – Quadrupole
Time of Flight
QTRAP® – Triple Quad Linear
SWATH® – Data Independent
SelexION® – Differential
MicroLC – Microflow
View All Technology
From substantiating new discoveries through
to end-to-end validated workflows for high-throughput biomarker
in the clinic, thousands of scientists worldwide depend upon mass
innovation from SCIEX to advance their work in the fields of
research, omics and diagnostics.
View All Clinical
Pesticides & herbicides
Soil and biota
View All Environmental
How do you protect your reputation and meet today’s global food
standards? Whether you are a commercial lab or a food manufacturer,
quality of your food testing data is vital to your business. SCIEX
solutions help you meet maximum residue limits (MRLs) with
data that you can genuinely count upon. With a portfolio of
applications, your lab can quickly and easily react to diverse
Authenticity & Profiling Analysis
& Food Contact Substance Analysis
Food and Beverage Testing
How do you ensure the integrity of your
results in an industry that is never constant? By accurately
even the smallest compound angles you can deliver evidence that
SCIEX forensic analysis solutions deliver fast, highly accurate
data across a multitude of compounds and biomarkers, from the known
the new and novel.
View All Forensics
and SNP Analysis
View All Life
Transform the capacity and capability of your biologics pipeline
complete end-to-end solutions that make your lab more productive,
more successful. With a longstanding track record in pharma
development and manufacturing, our unparalleled application
with best-in-class hardware, software and support all integrate to
revolutionize your lab.
Native Mode Analysis
Nucleic Acid and Plasmid Analysis
View All Pharma
Mass Spec Operators
View All Diagnostics
The SCIEX Now Learning Hub offers the most diverse and flexible learning options available, with best-inclass content that helps you to get the most out of your instrument and take your lab to the next level. Available personalized learning paths based on the latest memory science ensure better knowledge retention, and automated onboarding and enrollment means you’ll get up and running faster.
SCIEX Now Learning Hub offers the most diverse and
flexible learning options available, with best-in-class content that
helps you to get the most out of your instrument and take your lab
the next level. Available personalized learning paths based on the
latest memory science ensure better knowledge retention, and
onboarding and enrollment means you’ll get up and running faster.
SCIEX Learning Manager provides you with the infrastructure to assign, monitor and report on your staff's
competency through a single digital platform. Effectively manage the training process for new hires, ensure
continuous staff development and access information with a single log-in to your SCIEX account.
You can browse, filter, or search our
extensive list of training offerings. Choose from over 100
eLearnings or search for an instructor-led course near you. Once you
select the course you want to take, you will be directed to
Learning Hub for enrollment (login required).
SCIEX Now Online is the Everything, Anytime
destination for all your SCIEX support needs. You can keep track of
activities that matter most to you and manage your lab in the most
efficient way possible. Extensive self-help resources like our deep
Knowledge Base, enable you to solve many problems on your own. SCIEX
is available 24/7 and your new SCIEX instruments are automatically
to your profile when you purchase.
SCIEX Now Learning Hub
Manage My Instruments
SCIEX Now New Feature Request
Software New Feature Request
Online ordering solutions
Don't have an
account? Create One
Visit your SCIEX
No one understands your needs and can support
your systems better than we can. Our mission is to help you be
successful, whether it's to repair your instrument, assist with your
workflows, or help you maximize productivity in your lab. Whatever
challenge, global SCIEX Service and Support personnel are subject
experts who are focused on mass spec and capillary electrophoresis,
you'll be able to achieve your scientific goals quickly and
Plus Suite for Your New LC-MS
Instrument Service & Support
View All Support Tools
SCIEX Now Learning Hub is much more than online
courses. For the most comprehensive option, you can select Success
Programs: personalized, blended online and in-person courses. If
needs are met by a visit from one of our training experts, you can
choose multiple Onsite Training days to get your lab running. Visit
SCIEX training center in North America, Europe or Asia for intensive
classroom and laboratory training. And your online courses are
any time, from anywhere, right here.
Login to SCIEX Now Learning Hub
Success Programs at Your
Scientist Training at Your Site
Visit all Training
If you have CE, LC or mass spec questions,
then SCIEX has the answers. SCIEX support is the single destination
your system questions. We aim to fully assist you with virtually
application of our instruments, helping you to get the most out of
lab resources and assets.
View All Request Support
As a life science researcher, you need the
tools and support to help you create the scientific foundation in
pursuit of expanding the knowledge-base, whether it is understanding
fundamental biology, finding new biomarkers, discovering ways to
our quality of life, or other areas of research. We are committed to
same goals and put the very same dedication into our work to help
address your most significant research challenges.
View All Partnership
Regulatory compliance is as paramount for us
as it is for you. That’s why we have made it easy for you to freely
reference all relevant technical and product regulatory documents.
give you confidence that, with SCIEX, you will fully comply with
legislation, adhere to your laboratory protocol and meet industry
Declaration of Conformance
Safety Data Sheets
Certificates of Analysis
View All Regulatory Documents
SCIEX supplies extensive documentation to help you prepare for, use, and maintain your SCIEX hardware and software products, and we update this documentation regularly. On the Customer Documents page, you can search for and download the latest documents for your product.
Join the SCIEX community today to
interact with your peers, share and exchange ideas, develop
your knowledge, stay up-to-date with the latest products,
post insights and questions, comment on others and receive
support. This community is designed to help you, our
customers, move science forward and get the answers you
need. We’re committed to engaging with and listening to you,
to create the best customer experience possible and to
contribute to the success of your work.
Environmental / Industrial
Food and Beverage
Life Science Research
Now Feature Requests
View All Community
Our favorite papers
Meet our executives
In the news
You've got questions. We've got experts who
can help. Contact us to find out more, talk to a specialist, explore
solutions or get expert support.
Request a quote
SCIEX success network
Request hosted catalog
Global public relations
Bosnia and Herzegovina
United Arab Emirates
United Republic of Tanzania
AB Sciex is doing business as SCIEX. © 2010-2018 AB Sciex. The trademarks mentioned herein are the property
of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman
Coulter® is being used under license. Product(s) may not be available in all countries. For
information on availability, please contact your local representative. For
research use only. Not for use in diagnostic procedures.
Download tech note (PDF)
Using the ZenoTOF 7600 system, powered by SCIEX OS Software
ZenoTOF 7600 system technical overview, providing an overview of the hardware innovations and some application examples. Highlights include increased sensitivity with Zeno MS/MS, with > 90% duty cycle across the entire MS/MS mass range, and electron activated dissociation (EAD) for tunable free electron-based dissociation for added flexibility during compound identification.
The key to achieving robust analytical results lies in the combination of sensitivity, selectivity, and specificity. Sensitivity ensures there is plenty of signal to identify and quantify analytes of interest. Selectivity differentiates analyte signal from noise and interferences. Specificity ensures compound identifications are accurate and confident. The technological advancements in the ZenoTOF 7600 system combine qualitative flexibility and quantitative power for the most demanding sample types and workflows.
A hybrid collision cell is at the heart of the technological advancements in the ZenoTOF 7600 system. Previously, QTOF mass spectrometers have suffered from duty cycle losses as a result of mating time-of-flight (TOF) analysis, a pulsed measurement technique, with the continuous beam coming from the quadrupole ion path. A series of ion-staging events and reverse-mass sequential ion release, with high-capacity ion traps, allow for duty cycle losses to be mitigated and for MS/MS sensitivity gains of 4-20x.1 The cell also has the ability to perform both collision induced dissociation (CID) and electron activated dissociation (EAD) experiments for high-resolution MS/MS flexibility. Electron kinetic energies can be precisely tuned from 0-25 eV without the use of chemical transfer reagents. This tunability means EAD can be performed on a wide array of analytes, from multiply-charged peptides to singly-charged small molecules.2 The ability of the EAD cell to contain a high density of electrons allows for rapid reaction rates that keep up with fast chromatographic separations.
Figure 1. EAD MS/MS spectra of melittin with and without the Zeno trap activated. MS/MS spectra enhanced by using the Zeno trap (top, blue) shows 5-10x improvement in sensitivity across the mass range compared to the EAD MS/MS spectrum acquired without the Zeno trap activated (bottom, pink), with negligible changes to noise. EAD yields significant sequence coverage for structural elucidation.
Quadrupole time-of flight instruments most commonly use the orthogonal injection of ions coming from a quadrupole collision cell into the flight tube region, because this configuration maximizes TOF resolution, mass accuracy, and sensitivity for an entire spectrum without the need for scanning. This type of ion pulsing, however, suffers from a relatively low duty cycle. Typically, only 5-25% of ions are ejected with each pulse of the accelerator, depending on the geometry and m/z range. This is not usually an issue in the MS1 dimension, because the ion current as generated by modern sources (such as the Turbo V ion source), and transmitted by modern ion capture technology (such as the QJet ion guide), results in ion currents that need to be reduced to prevent saturation and to protect the longevity of TOF MS detectors. In the MS/MS dimension, however, an improvement in the duty cycle can lead to significant gains in sensitivity.
The ion losses are a result of the drift region between the collision cell and the TOF accelerator. This region behaves as a crude TOF separation, where low m/z ions migrate faster than high m/z ions and, as a result, a significant fraction over or under migrate to the accelerator region and are lost with each pulse. Previously, there have been many attempts to overcome this lack of synchronicity. It has only been achieved, however, either for narrow mass ranges or at low acquisition frequency.
Use of the Zeno trap overcomes these technological barriers to recover duty cycle losses across the entire m/z at up to 100 Hz acquisition frequency. This is achieved using a linear ion trap, referred to as a Zeno trap, at the exit of the collision cell. The mechanism of trapping and releasing ions is highlighted in Figure 2. Ions enter the ion trap and are contained with potential barriers on the ZG and IQ3 lenses, while subsequent packages of ions are accumulated in the LINAC collision cell, preventing ion loss. The trapped ions are left to energetically cool and are subsequently released based on potential energy resulting in an ordered release generally ranging from high m/z to low m/z. In this way, each ion across the mass range reaches the center of the TOF accelerator simultaneously.
Figure 2: Timing diagram of gating voltages, AC ramp and TOF acceleration pulses. Ions are accumulated in a small ion trap at the exit of the collision cell, then released in reverse mass order to perfectly synchronize with each accelerator pulse.
This simple trapping and releasing mechanism leads to significant gains in MS/MS sensitivity, as highlighted in Figure 3. MS/MS with the Zeno trap activated results in a 4- to 15-fold (or greater) gain in signal, with increased gains at low m/z fragments. The Zeno trap efficiency combined with precise ion-release timing yields ≥90% of the theoretical gain across the entire mass range. Due to the degree of selectivity afforded with high resolution MS/MS data, these improvements in signal are combined with negligible changes to noise, resulting in spectral and chromatographic signal-to-noise on the order of the gains observed in raw signal (Figure 4, 5).
These improvements in MS/MS sensitivity not only have the ability to drastically improve LOQs for quantitative assays, but this additional sensitivity can be used to revolutionize entire workflows. With the Zeno trap activated, high-quality MS/MS spectra can be used for confirmation, identification, or library matching at much lower mass loading. This gives the ability to significantly dilute precious samples and improve ionization efficiency by minimizing matrix effects and improve instrumental robustness with lower mass loading. At the same sample loading, MS/MS with the Zeno trap on yields greater confidence in identifications while allowing for the ability to discover new metabolites, peptides biomarkers and contaminants at lower concentrations than ever before.
Figure 3: Theoretical sensitivity gains in MS/MS as a function of fragment m/z and acquisition mass range upper limit using the Zeno trap. Sensitivity gains are the result of the recovery of duty cycle losses that are a natural result of mating TOF analysis, a pulsed measurement technique, with the continuous beam coming from the quadrupole ion path. Greater ion losses occur as the upper limit of the MS/MS scan range increases. Zeno trap technology has the ability to recover >95% of these losses.
Figure 4: Sensitivity gains with the Zeno trap for difenoxuron with MRMHR acquisition. Due to the selectivity afforded by MRMHR, the gain in signal from the Zeno trap is accompanied by a minimal gain in noise. (Left) A 13-fold intensity gain results in a 12-fold signal to noise gain for the m/z = 72.044 fragment of difenoxurion. (Right) All peaks in MS/MS spectra show a sensitivity gain (6-13 fold) with use of the Zeno trap.
Figure 5: Sensitivity gains with the Zeno trap for SPYVITGPGVVEYK from PepCalMix with MRMHR acquisition. (Left) A 5.65-fold gain in peak area for m/z = 1070.50 fragment ion with a 5.34-fold gain in S/N. (Right) Gain observed for higher m/z peptide fragment ions is 5-7 fold with the Zeno trap on across the MS/MS mass range.
Tandem mass spectrometry is dominated by collisional induced dissociation (CID) that generates ion fragments for quantification and identification of molecular species. With CID, ions are generated in an accelerated cell filled with a neutral gas species promoting molecular collisions that result in bond cleavage, typically at the most labile sites. Although fast and efficient, CID can often result in few diagnostic fragments that are insufficient to elucidate structural information from unknown features or to differentiate isomeric species.
Electron activated dissociation (EAD), conversely, describes a family of free electron-based dissociation mechanisms characterized by the charge state of the precursor ion and the kinetic energy of the electron beam. EAD mechanisms are known to give complementary information to CID. The extent and location of bond cleavage differs for radical (EAD) and thermal (CID) techniques.
The ZenoTOF 7600 system features the EAD cell, a new electron beam optic design that simultaneously and independently traps precursor ions and free electrons for efficient radical fragmentation. With the EAD cell, the system has the ability to tune electron energies above zero, which opens up radical fragmentation to applications beyond biomolecules. Tunable electron kinetic energy from 0-25 eV provides access to different fragmentation regimes (Figure 6), including electron capture dissociation (ECD), hot ECD, and electron impact excitation of ions from organics (EIEIO). These advances enable fast, precise and quantitative dissociation of various analytes ranging from singly charged small molecules to multiply protonated proteins. Also, at higher energies, reaction times are reduced allowing for these dissociation techniques to be used on a chromatographic timescale. It is the combination with the Zeno trap that allows EAD to now have the sensitivity and specificity needed for routine use.
Figure 6: EAD family classified by precursor species and the kinetic energy of the electron beam. Common electron-based dissociation techniques and their typical applications are listed.
Post-translational modifications (PTMs) are widely important for various protein functions, including protein conformation, signaling and activity. Some PTMs can be difficult to characterize using mass spectrometry, however, when they are labile, CID is used as the dissociation technique. Radical dissociation techniques have the ability to maintain these PTMs, which allows for peptide backbone mapping, while simultaneously elucidating the identity and location of the PTM. Figure 7 shows the example of a phosphorylated peptide, LITV, using hot ECD (KE = 7 eV). Here, not only is nearly the entire peptide sequenced with hot ECD, but the location of the phosphorylation is maintained.4
Figure 7. Phosphorylated peptide, LITV, analyzed using EAD-MS/MS. (Top) LITV EAD-MS/MS spectrum with Zeno trap on. (Bottom) LITV EAD MS/MS spectrum with Zeno trap off. MS/MS sensitivity is significantly enhanced with the Zeno trap activated. With the Zeno trap on, 100% sequence coverage is achieved with phosphorylation site location confirmed on multiple c- and z- ion series.
Having the ability to tune electron kinetic energies in the 5 to 15 eV range opens up EAD to the realm of singly charged molecules. Electron capture, resulting in neutralization, is reduced at this kinetic energy, allowing the electrons to induce dissociation through radical mechanisms. Figure 8 shows the comparison between CID and EIEIO for the fragmentation of a sphingomyelin lipid species. EIEIO gives spectral information for nearly every bond to elucidate head group identity, backbone type, carbon chain lengths, double bond positions and double bond stereoisomerism. In a similar fashion, EIEIO can be used to differentiate between isomers of small molecules. Figure 9 highlights the unique, characteristic fragment for O-glucuronide conjugated darunavir compared to its N-glucuronide isomer, allowing for more precise molecular information within metabolite identification workflows. EIEIO fragmentation also opens up additional specificity for non- targeted and suspect screening workflows. Figure 10 highlights the difference between EIEIO and CID for the fragmentation of azoxystrobin, a fungicide. The CID spectrum is dominated by two main fragments, whereas the EIEIO spectrum contains over 200 peaks with S/N >10, which allows for significantly improved confidence during library matching and structural elucidation.
Figure 8: Comparison between dissociation product spectra by EIEIO (A) and CID (B). The sample was a synthesized standard SM, SM(d18:1,12:0).
Figure 9: EAD (top) and CID (bottom) spectra of O-glucuronide conjugated darunavir. EIEIO creates unique fragments characteristic of the glucuronide conjugation positioning.
Figure 10: Comparison between CID and EAD MS/MS spectra for azoxystrobin. EIEIO (middle top, blue) creates roughly 100x the number of fragments with S/N >10 compared to CID (middle bottom, pink). The XICs for EAD (left) and CID (right) highlight the flexibility of EAD for ion ratio-based confirmation.
LDR is important in many applications where the analyte concentration varies widely. The ZenoTOF 7600 system is equipped with a 4-channel MCP detector with ADC signal processing, resulting in greater than 5 orders of inter-scan linear dynamic range in both positive and negative ion modes, and for both MS and MS/MS acquisitions (Figure 11). Further, the ADC detector is capable of 4 orders if intra-scan linear dynamic range, allowing for the simultaneous detection of both high and low level analytes without a loss of data quality.
Figure 11: Linear dynamic range of the 4-channel NCP detector with ADC signal processing. Greater than 5 orders of inter-scan linear dynamic range can be achieved in both positive ion (top) and negative ion (bottom) modes, for both MS (left) and MS/MS (right) acquisitions.
The ZenoTOF 7600 system comes equipped with a high-speed LINAC collision cell that allows for fast acquisition rates, suitable for highly multiplexed experiments and rapid LC separations. Combined with the high speed of the MCP detector, the ZenoTOF 7600 system is capable of >100 Hz acquisition rates, with accumulation times as low as 5 msec, without sacrificing mass accuracy and resolution. Figure 12 highlights the maintenance of mass accuracy, mass resolution and peak intensity across a range of accumulation times.
Figure 12: Peak intensity, mass resolution and mass accuracy across accumulation times. Faster accumulation times do not come at a sacrifice of mass resolution or mass accuracy with the ZenoTOF 7600 system. Mass resolution is consistent across the entire mass range.
The ZenoTOF 7600 system has the option for the OptiFlow Turbo V ion source, which is designed for microflow and nanoflow chromatography (Figure 13).5 Probe and electrode combinations are pre-optimized for sensitivity and robustness from 0.1 to 200 µL/min. This means no manual adjustments are needed to maximize performance. SecurityLink tubing and fittings are used such that all fittings are finger tight, leak free and have zero dead volume. For microflow chromatography, the exit of the column connects directly to the electrospray probe, minimizing post-column broadening for enhanced S/N. The instrument comes configured with the OptiFlow interface, so switching between high flow or microflow to nanoflow is a toolless change, without the requirement of breaking vacuum on the system.
Figure 13: OptiFlow source configurations. (Left) Microflow configuration. (Right) Nanoflow configuration.
The Destination for All Your Support Needs