TripleTOF® 5600+ System

Setting the QTOF standard for high performance qualitative and quantitative analysis on a single platform

The TripleTOF 5600+ System combines the best-in-class front end of SCIEX Triple Quad™ technology with the Accelerator TOF™ Analyzer, to deliver nominal mass and accurate mass benefits in a single platform.

This proven workhorse uniquely integrates comprehensive, qualitative exploration, rapid profiling, and high-resolution quantification workflows, providing high performance and versatility for pharmaceutical development, omics research and any challenging unknown compound analysis.

Find out more from every sample

  • Confidence in comprehensive coverage with complete and unbiased SWATH® Acquisition
  • Reliability and maximum uptime with stable mass accuracy for over 50 hours of continuous LC-MS operation
  • Sensitive, high-resolution system – with low limits of quantitation (LOQ) equivalent to high-performance triple quadrupoles
  • Suited for regulated environments to support 21 CFR Part 11 compliance
 TripleTOF 5600+ System
Key features

Enhanced selectivity with differential ion mobility

Get more out of every sample with an additional dimension of separation with SelexION® Differential Ion Mobility Technology.

Robust spray with the DuoSpray™ Turbo V Source

Efficient ion production while limiting contamination for maximized robustness, reliability and uptime.

Comprehensive coverage with data-independent SWATH Acquisition

Variable window capabilities allow for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples.

Maximize flexibility with front end separations

Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow, and capillary electrophoresis spray ionization (CESI).

High resolution accurate mass workhorse

Dig deeper in your sample

With optimized ion production, focusing, reflection and detection, the TripleTOF 5600+ System will help you dig deeper into complex samples.

IonDrive™ Turbo V Source

The new design of the legendary Turbo V Source increases ion production by using enhanced gas flow dynamics and optimized heater configuration to improve reliability, reproducibility, and robustness at higher flow rates.

QJet® Ion Guide

Designed for robustness, the QJet Ion Guide provides a large capture radius for efficient transfer of ions from source to quadrupole optics.

High frequency LINAC® Collision Cell

High drive frequency collision cell provides better ion transmission, higher duty cycle, and improved resolution by focusing ions prior to entering TOF.

Accelerator TOF Analyzer

Time-of-flight analyzer uses a 2-stage mirror and 15 kV acceleration to improve resolution and efficiency. Get the speed and sensitivity required for triple quadrupole like quantification, but with high resolution and mass accuracy for qualitative applications.

Detection system

4-channel MCP detector with TDC processing technology provide more than 4-orders of linear dynamic range while improving robustness and lifetime at high ion currents.

Key features  
Mass range TOF 40 kDa
Mass selection range Q1 5 – 1250 m/z
TOF-MS mass resolution and acquisition speed ≥35,000 (FWHM) at 956 m/z, using 50 ms accumulation time
TOF-MS/MS mass resolution and acquisition speed ≥25,000 (FWHM) at 195 m/z, and ≥18,000 (FWHM) at 86 m/z, using 50 ms accumulation time
Detector type 4-channel MCP detector with TDC signal processing for extended dynamic range
Flexible data acquisition workflows TOF-MS
Information dependent acquisition (IDA) MS/MS
Data-independent SWATH Acquisition with fixed or variable Q1 isolation windows 
Targeted MRMHR workflow
Ionization sources IonDrive Turbo V, DuoSpray™ source, each with TurboIonSpray® or atmospheric-pressure chemical ionization (APCI) probes available; Digital PicoView NanoSource
Orthogonal front-end separation compatibility Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow chromatography, capillary electrophoresis spray ionization (CESI), or using SelexION Technology
Variable window SWATH Acquisition Data-independent SWATH Acquisition with variable window capabilities, allows for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples. Read More
Data file portability Condensed data file sizes simplify and streamline data storage and portability for sharing with colleagues, without losses in data quality, completeness or security

Explore the unique TripleTOF System technology


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SelexION Technology for TripleTOF Systems Site Planning Guide (JA)

SelexION Technology for TripleTOF Systems Site Planning Guide (ZH)

SCIEX LC-MS-MS Application Technology on Origin Identification of Dairy Products based on Alpha-lactalbumin and Beta-lactoglobulin

Application of Unbiased SWATH Acquisition in the Qualitative Analysis of Rubber Plug Extract of Drug Packaging Materials

Rapid Characterization of the Cow Milk and Human Breast Milk Lipidome using High Resolution Mass Spectrometry X500R System

Qualitative and Quantitative Analysis of Heterocyclic Amines in Hot Processed Meat Products by SWATH® of X500R

Application of TripleTOF 5600 System in the Identification of Components of Tibetan Drug Rhodiola rosea L

Stability Evaluation of MicroFlow-SWATH Serum Proteomics Strategy Based on Eksigent M5 Liquid Chromatography

TripleTOF 5600 system combined with Natrual Products Library quickly identify Psoraleae components

A metabolomics approach for authentication of Ophiocordyceps sinensis by 5600+ mass spectrometry

Metabolomic Approach for the Authentication of Berry Fruit Juice by TripleTOF 5600+

Application of SWATH Based Quantitative Proteomics in Biological Study


Abstract-Analysis of components in Shengmai injection by CESI-MS

The Application of TripleTOF 5600 System for Identification of Chemical Components in Polygonum Cuspidatum

Identification of Extractable and Leachable in Single Use Packaging of Drug on the X500 QTOF System

Analysis of glycopeptides in erythropoietin by CESI-MS/MS and NanoLC-MS/MS techniques

Quickly identification of phase II metabolites in plasma after oral Compound Traditional Chinese Medicine by SCIEX TripleTOF system software

Fast Efficient Disulfide Bond Mapping Using BioPharmaView Software

Study on Metabolomics and Lipidomics of Chiral PCB 91 in Earthworm

Precision Medicine Research of Lung Adenocarcinoma Based on MicroFlow-SWATH Proteomics Strategy

DuoSpray Sorgente di ionizzazione per sistemi TripleTOF® Guida per l'operatore

User Guide: DuoSpray Ion Source for TripleTOF Systems Operator Guide (FR)

SCIEX vision Journal Issue 4

Catalogue of Literatures on SCIEX SWATH technology (Volume 1)

Catalogue of Literatures on target lipidomics using Triple Quad QTRAP mass spectrometry (Volume 1)

Catalogue of Literatures on Metabolomics using SCIEX mass spectrometry (Volume 1)

Catalogue of Literatures on untarget lipidomics using SCIEX high resolution mass spectrometry (Volume 1)

Catalogue of Literatures on Traditional Chinese Medicine (Volume 1)

TripleTOF® 5600+ LC-MS/MS System One System, Flexible Workflows

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PowerPoint Presentation

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Microsoft PowerPoint - Pfizer_5600 mAb poster_June 2014 [Read-Only] [Compatibility Mode]

48x72 poster template

High-Throughput and Automated Software Workflow Strategies for Small Molecule Identification and Characterization Using a High Resolution Q-TOF Mass Spectrometer

Slide 1

Automated and Rapid Characterization of Low-Molecular-Weight Heparin Oligosaccharides Using TripleTOF 5600 Mass Spectrometer with SimGlycan Software

Multi-Omics Analysis of hESC Neural Differentiation

Improved Metabolite Identification using Data Independent Analysis for Metabolomics

Automating Protein Digestion for Reproducible Proteomics

Investigating Biological Variation of Liver Enzymes

DMS Resolves Adducts of Phosphatidylcholine (PC)

Comprehensive Quantification with Qualitative Confirmation using the TripleTOF 5600 System

Identificación Exacta en Análisis Forenses

TripleTOF Environmental Profiling_AB SCIEX_0460210-02.docx

Folie 1

Separation of Ephedrine and Pseudoephedrine Isomers Using Differential Mobility

Perfeccionando la Identificación de Metabolitos Utilizando una Adqusición Independiente de los Datos para Metabolómica

AB SCIEX Master Your Data to Get to the Answer, View Results with Ease and Confidence - Targeted Screening

Comprehensive Phosphoproteomic Quantitation using SWATH® Acquisition

Differentiation of Sialic Acid Linkages using Differential Mobility

Simplifying the Use of Ion Libraries During Data Processing of SWATH® Acquisition Proteomics Data

High Throughput, Data Independent Acquisition for Qualitative and Quantitative Shotgun Lipidomics

iTRAQ Reagents with Cysteinyl Peptide Enrichment for Protein Profiling

Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances

Resolution of Ether- and Diacyl-Linked Phospholipids by DMS

Comprehensive Quantification with Qualitative Confirmation using the TripleTOF 5600 System

Small Molecule MS Imaging of an Italian Bank Note

Extending Metabolome Coverage-untargeted Metabolomic Workflows

AB SCIEX TripleTOF 5600 Non-Targeted Screening of an unlimited number of contaminants

Confident Identification For Comprehensive Screening

Characterization of 2-AB Labeled Released N-linked Glycans Using SCIEX TripleTOF® 5600+ LC-MS/MS System

MSMSall with SWATH Acquisition

SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids

Rapid Identification and Quantification of Novel Psychoactive Substances in Human Whole Blood Using SWATH® Acquisition

Automated Targeted Screening of Hundreds of Metabolites - Using an Accurate Mass Metabolite Spectral Library with SCIEX OS Software

Microsoft Word - Heparin_technote_Annuv1.docx

ProteinPilot Descriptive Statistics Template

Proteome Wide Quantitation of Mycobacterium Tuberculosis

Non-Targeted Metabolic Profiling of Human Urine Samples

Wide Compound Coverage for Confident Analyte Identification Using SWATH® Acquisition on the SCIEX TripleTOF® and X-Series QTOF Systems

Don’t Miss Any Novel Psychoactive Substance in Your Sample Using the Wiley MMHW LC-HR-MS/MS Spectral Library

Dont miss Synthetic Drug Present in Sample

Impact of High-Resolution MRM Analysis on Complex Proteomes

Improved Data Quality Using Variable Q1 Window Widths in SWATH Acquisition


Analysis of Intact MUPs (Major Urinary Proteins) from Mice by CESI-MS

Automated Trypsin Digests for Reproducible Peptide Map Analysis

MetabolitePilot Software 2.0

Rapid Metabolite Identification using MetabolitePilot™ Software and TripleTOF™ 5600 System

Digitizing the Proteomes From Big Tissue Biobanks

Broader Coverage and Automatic Mass Calibration Using the TripleTOF™ 5600 System with DuoSpray™ Ion Source