Live webinar
Advancing de novo sequencing in proteomics with ZenoTOF 7600 system, Bio Tool Kit software, and PEAKS Studio
May 28, 2025 | 1:00 p.m. AEDT | 10:00 a.m. SGT
Why de novo sequencing still matters in proteomics
- While shotgun proteomics using database searching remains the workhorse of protein identification, there are growing needs that it can’t fully address
- De novo sequencing remains essential when:
- No reference database exists (e.g., non-model organisms, microbiomes)
- PTMs, mutations, or sequence variants are present and unknown
- It is also a powerful complement to database searching, adding depth and novelty to discovery workflows
In short: if the database can’t tell you the answer, de novo might
Key learning points:
- Understand the value of de novo sequencing in modern proteomics workflows
- Recognize scenarios where de novo adds value beyond database searching
- Learn a practical workflow: from feature detection (MarkerView) → sequence inspection (BioToolKit) → automatic sequencing (PEAKS)
- See how EAD fragmentation improves confidence in de novo results
- Gain tips on how to optimally use different tools to investigate more deeply
Speaker
Jeremy Potriquet, Field Applications Specialist, SCIEX
Jeremy has extensive knowledge of peptide and protein analysis and quantification from previous roles as a scientific officer and proteomics manager. He is experienced in processing a variety of sample matrices ranging from cancer-related matrices to those for infectious diseases covering the study of exosomes, parasites, venoms and lately immunology. Mass spectrometry has always been an essential part of Jeremy's career and with SCIEX as a Field Application Specialist. He applies his knowledge to provide support and guidance for the numerous SCIEX technology users in Australia and New Zealand (ANZ).
The webinar is over. The on-demand version will be available soon.