AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.
NanoLC 400

NanoLC 400

NanoLC 400

NanoLC 400

For research use only. Not for use in diagnostic procedures.

The NanoLC 400 system is the latest generation in NanoLC from SCIEX and offers the widest flow rate range of any SCIEX NanoLC system to date, enabling a diverse collection of workflows on a single low flow LC/MS system. Interchangeable flow control modules enable users to switch effortlessly between nanoflow and microflow applications. Proteomics researchers no longer have to choose between the high sensitivity of Nano-LC/MS and the high throughput of micro-LC/MS. Now one system can be used to move from protein ID and discovery work to higher throughput targeted assays.

With improvements to SCIEX's proven Microfluidic Flow Control, the NanoLC 400 and MFCPlus technology not only enables the simple interchange between different flow rate ranges, but also ensures reliable gradient delivery and reproducible retention times. This is critical for getting the most out of data-independent strategies for quantitative proteomics like SWATH® Acquisition on an SCIEX mass spectrometer and any quantitative proteomics workflow.

The NanoLC 400 system is available in single- and dual-gradient configurations in the same compact footprint; and integration with the cHiPLC system and popular mass spectrometry software packages* makes this system a universal front end to almost any nano-LC/MS system, enabling a broad range of workflows for busy multi-user and multi-instrument proteomics laboratories.

*Direct control through SCIEX Analyst software, and Thermo Xcalibur.

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Flexibility for Today’s Proteomics

The NanoLC™ 400 product series enables the full range of protein expression workflows that drive the identification of novel biomarkers to advance precision medicine research.

Ideal for the biomarker researchers digging deep in discovery or scaling up to validate their research findings, this front-end system is an innovative, flexible solution for a broad range of workflow needs.

Plug-and-play flow cartridges give you the flexibility to easily switch from the ultimate sensitivity of nanoflow LC-MS for discovery to high-throughput microflow LC-MS for validation.

The Choice is Yours

The NanoLC 400 Series is available in two models, with patented pumps that make these systems among the most accurate nanoflow models on the market. Combined with industry-leading SCIEX mass spectrometers, the NanoLC Series provides high sensitivity, robustness and ease of use to support the latest in data-independent quantitative proteomics with SWATH® Acquisition.
NanoLC 415:
When your sample is limited, and sensitivity is needed choose the NanoLC 415 for direct-to-column or trap-and-elute workflows. The NanoLC 415 is a complete package, providing an autosampler, solvent organizer, sample storage and cooling; plus a column oven - giving you the confidence that you will be up and running quickly.
NanoLC 425:
The NanoLC 425 is an ideal HPLC system for labs performing a broad range of proteomics analyses. The additional pump gives you the power to dive deeper into your sample – with the ability to see more using 2D workflows. With the flexibility to have pre-set nano and microflow configurations, this 2-in-1 system is ideal for labs with diverse applications that need to actively switch between flow regimes. Like the NanoLC 415, this integrated system includes an autosampler, solvent organizer, sample storage and cooling; plus a column oven – helping your lab to realize its full potential.
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Meeting the Needs for Every Proteomics Workflow

With single-gradient and dual-gradient models available - workflows from simple trap and elute to complex multi-dimensional separation are possible. The NanoLC 400 Series readily fits the ever-changing needs of your proteomics lab.

nanoLC400

When Samples Are Precious

When you only have minimal amounts of sample, the NanoLC 400 Series can get you the high-quality identification and quantitation data you need for new biological insights.

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nanoLC400

Industrialize your workflow

When you are ready to industrialize your workflow, the NanoLC 400 Series can readily move to microflow to give you more robustness and higher throughput.

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sensitivity

Excellent Separations Every Time

With the NanoLC 400 Series’ patented Microfluidic Flow Control™ technology, you get consistent, accurate results without flow splitting, even at the high pressures.

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Built For Simple or Complex Workflows

Key Features of the NanoLC400 Series
Models NanoLC 415 for basic direct-to-column or trap-and-elute workflows
NanoLC 425 for more advanced 2D workflows
Column Oven Column oven provides excellent retention time reproducibility by ensuring consistent column temperature
Flow Rates Multiple flow rate options with user-interchangable plug-and-play flow modules to switch between nanoflow (100 nl/min - 1 µl/min) and microflow (1 - 10, or 5 -50 µl/min)
Flow Accuracy SCIEX's patented Microfluidic Flow Control technology, for consistent, accurate retention times without flow-splitting
Software Integrated and controlled with SCIEX Analyst software

Learning Center

Replace the Syringe

Manual Prime

Replace the Buffer Tubing

Replace the 10-port Rotor Seal

Replace the Piercing Needle Assembly

Replace the Piercing Needle

Replace the 6-port Rotor Seal

Replace the Sample Needle

Replace the Sample Loop

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Better quality data for your lab is just a click away. Consult with us to see how nanoflow can help you overcome your biggest challenges.

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RUO-MKT-18-2028-C
For research use only. Not for use in diagnostic procedures.

Overview
FeatureBenefit
Switch easily from nanoflow to microflow with interchangeable flow modules Switch easily from nanoflow to microflow with interchangeable flow modules

Click to Enlarge


 		 Shorter run times and higher throughput can be achieved using microflow instead of nanoflow. Plug & play modules enable effortless switching, and a faster autosampler supports the move from biomarker discovery to verification assays.
Retention time reproducibility Retention time reproducibility

Click to Enlarge

For quantitative proteomics, maintaining retention time reproducibility is crucial.

The latest in SCIEX’s Microfluidic Flow Control technology, MFC Plus™, uses fast flow measurement in combination with automatic feedback to generate accurate and reproducible flow rates without flow splitting. The result is gradient reproducibility typically better than 0.35% at flow rates as low as 100 nl/min.

To achieve the best possible retention time reproducibility, an optional column oven capable of temperatures up to 60°C is available for all nanoLC 400 systems. Elevated temperatures can also be employed to decrease column pressure and improve separation efficiency.

For advanced applications like sample pre-concentration or dual-column set-up, the SCIEX nanoLC 400 autosampler accommodates one or two 10-port nano valves rated at 10,000 psi and have an extremely low port-to-port volume of < 25 nl.

The system’s control software enables users to rapidly create experiments and modify methods. Its method editing wizards and built-in diagnostic tools provide ease-of-use to help maximize uptime.

RUO-MKT-18-2028-B

Specifications
FeatureNanoLC 400
Dimensions 22” x 26” x 9” (56 x 66 x 23 cm) (W x D x H)
Weight 57 pounds (26 kg)
Working temperature 15°C to 30°C
Maximum pressure 10,000 psi
Models Available NanoLC 415 1 loading pump plus 1 gradient pump
NanoLC 425 1 loading pump plus 2 gradient pumps
Flow rate ranges
Loading pump 1-50 μL/min
Gradient with nano flow module 100-1000 nL/min (up to 2 μL/min at reduced maximum pressure)
Gradient with low micro flow module 1-10 μL/min (up to 20 μL/min at reduced maximum pressure)
Gradient with high micro flow module 5-50 μL/min
Flow rate accuracy < 1% @ 500 nL/min with nano flow module installed
Retention time precision < 1% @ 500 nL/min with nano flow module installed and column at constant temperature (± 0.2 °C)
Gradient delay < 25 nl
Wetted parts stainless steel, titanium, PEEK, fused silica, FEP
I/O 1 x USB for instrument control each pump channel
2 x TTL in
8 x contact closure out
Instrument control Eksigent software for stand alone use
Eksigent software with drivers for Analyst® software (SCIEX)
Resources
Filter by Type:

Title Date
Microflow Chromatography-A Key to More Sensitive Met ID 05/24/2019
Digitizing the Proteomes From Big Tissue Biobanks 12/26/2018
Exploring the Sensitivity Differences for Peptide Quantification in the Low Flow Rate Regime 08/27/2018
Micro/Nano Columns Part List 08/21/2018
LC-MS-MS Solution for Determination of Nitrosamine Disinfection byproducts in Drinking Water 05/20/2018
Simplifying the Use of Ion Libraries during Data Processing of SWATH Acquisiton Proteomics Data 04/10/2018
Configuring NanoLC 425 for Nanoflow and Microflow LC-MS Workflows 12/20/2017
Using Multi-Omics Analysis to Study EMT Model of Prostate Cancer 06/04/2017
Proteome-wide Profiling of Drug Dose-Response in Prostate Cancer Cell Lines by Microflow SWATH Acquisition 06/04/2017
Dev of Hi-Throughput Proteomics Pipeline-SWATH Profiling Biochemical Individuality 06/04/2017
Impact of High-Resolution MRM Analysis on Complex Proteomes 05/02/2017
Achieving High Reproducibility for Quantitative Proteomics with Nanoflow LC-MS 05/01/2017
Assessing Impact of Extended Gradient Lengths for Microflow SWATH Acquisition 09/15/2016
NanoLC Systems for TripleTOF systems Test and Data Log 09/01/2016
Large-Scale Quantitative Measurements of Rat Brain Hippocampus Membrane Proteome in Prolonged Simulated Microgravity Model by 2D-SWATH Mass Spectrometry 06/06/2016
Accelerating Data Independent Acquisition with Microflow Chromatography 06/06/2016
Improving DIA Quantitation with microLC Using a Longer Gradient 06/06/2016
Host Cell Protein Analysis by Microflow-LC High Resolution SWATH-MS of Vaccine Samples Under Development 06/06/2016
Comprehensive Relative Quantification of the Cytochromes P450 by Micro-LC and SWATH Acquisition 06/06/2016
Declaration of Conformity for NanoLC 415 and NanoLC 425 06/01/2016
NanoLC 400-Configured for Microflow Flow Rates System Integration Test 06/01/2016
Industrializing Quantitative Proteomics Using Microflow LC and SWATH Acquisition 04/27/2016
Extending Depth of Coverage with SWATH Acquisition Using Deeper ION Libraries 12/10/2015
NanoLC 400 System - Replace the Piercing Needle 11/30/2015
M3 MicroLC System Overview - Sensitivity and Throughput for Precious Samples 11/01/2015
NanoLC 400 Systems Consumables and Accessories List 09/01/2015
Ultrafast, High Sensitivity microLC-MS/MS for Peptide Quantitation in Highly Targeted Assays 06/01/2015
User Guide: Ekspert nanoLC System Integration Tests 05/29/2015
Characterization of Intact and reduced therapeutic monoclonal antibodies using microflow size exclusion chromatography coupled with mass spectrometry 06/19/2014
Unlocking the Benefits of MicroLC with SCIEX and Eksigent Technology 06/17/2014
Ultra-Sensitive Quantitation of pg/mL Level Exenatide with Trap-and-Elute Micro-Flow LC/MS/MS 06/16/2014
Exploring microLC/MS/MS for Accelerating Peptide Quantitation Assays 06/15/2014
ekspert nanoLC 400 Systems Site Planning Guide 05/05/2014
Reproducible Analysis Whole Proteome Quantitation 01/06/2014
Reproducible Analysis for Whole Proteome Quantitation Using the Eksigent nanoLC 400 12/20/2013
Ekspert NanoLC 400 Systems Operator Guide 11/02/2013
On-line, Chip-Based Strategy for Increased Depth of Coverage in Proteomic Samples 05/03/2013
Achieve Low Flow Sensitivities with Micro Flow Chromatography on the QTRAP 6500 System for Targeted Quantitative Proteomics 05/01/2013
Brochure: Ekspert NanoLC 400 05/01/2012
Exploring the Sensitivity Differences for Peptide Quantification in the Low Flow Rate Regime
Simplifying the Use of Ion Libraries During Data Processing of SWATH Acquisition Proteomics Data
Microflow SWATH Acquisition for Industralized Quantitative Proteomics
Achieving Very High Reproducibility for Quantitative Proteomics with Nanoflow LC/MS
Configuring the NanoLC 425 System for Both Nanoflow and Microflow LC-MS Workflows
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AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.