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For research use only. Not for use in diagnostic procedures.
The NanoLC 400 system is the latest generation in NanoLC from SCIEX and offers the widest flow rate range of any SCIEX NanoLC system to date, enabling a diverse collection of workflows on a single low flow LC/MS system. Interchangeable flow control modules enable users to switch effortlessly between nanoflow and microflow applications. Proteomics researchers no longer have to choose between the high sensitivity of Nano-LC/MS and the high throughput of micro-LC/MS. Now one system can be used to move from protein ID and discovery work to higher throughput targeted assays.
With improvements to SCIEX's proven Microfluidic Flow Control, the NanoLC 400 and MFCPlus technology not only enables the simple interchange between different flow rate ranges, but also ensures reliable gradient delivery and reproducible retention times. This is critical for getting the most out of data-independent strategies for quantitative proteomics like SWATH® Acquisition on an SCIEX mass spectrometer and any quantitative proteomics workflow.
The NanoLC 400 system is available in single- and dual-gradient configurations in the same compact footprint; and integration with the cHiPLC system and popular mass spectrometry software packages* makes this system a universal front end to almost any nano-LC/MS system, enabling a broad range of workflows for busy multi-user and multi-instrument proteomics laboratories.
*Direct control through SCIEX Analyst software, and Thermo Xcalibur.
The NanoLC™ 400 product series enables the full range of protein expression workflows that drive the identification of novel biomarkers to advance precision medicine research.
Ideal for the biomarker researchers digging deep in discovery or scaling up to validate their research findings, this front-end system is an innovative, flexible solution for a broad range of workflow needs.
Plug-and-play flow cartridges give you the flexibility to easily switch from the ultimate sensitivity of nanoflow LC-MS for discovery to high-throughput microflow LC-MS for validation.
The NanoLC 400 Series is available in two models, with patented pumps that make these systems among the most accurate nanoflow models on the market. Combined with industry-leading SCIEX mass spectrometers, the NanoLC Series provides high sensitivity, robustness and ease of use to support the latest in data-independent quantitative proteomics with SWATH® Acquisition.
When your sample is limited, and sensitivity is needed choose the NanoLC 415 for direct-to-column or trap-and-elute workflows. The NanoLC 415 is a complete package, providing an autosampler, solvent organizer, sample storage and cooling; plus a column oven - giving you the confidence that you will be up and running quickly.
The NanoLC 425 is an ideal HPLC system for labs performing a broad range of proteomics analyses. The additional pump gives you the power to dive deeper into your sample – with the ability to see more using 2D workflows. With the flexibility to have pre-set nano and microflow configurations, this 2-in-1 system is ideal for labs with diverse applications that need to actively switch between flow regimes. Like the NanoLC 415, this integrated system includes an autosampler, solvent organizer, sample storage and cooling; plus a column oven – helping your lab to realize its full potential.
When you only have minimal amounts of sample, the NanoLC 400 Series can get you the high-quality identification and quantitation data you need for new biological insights.
When you are ready to industrialize your workflow, the NanoLC 400 Series can readily move to microflow to give you more robustness and higher throughput.
With the NanoLC 400 Series’ patented Microfluidic Flow Control™ technology, you get consistent, accurate results without flow splitting, even at the high pressures.
|Models||NanoLC 415 for basic direct-to-column or trap-and-elute workflows
NanoLC 425 for more advanced 2D workflows
|Column Oven||Column oven provides excellent retention time reproducibility by ensuring consistent column temperature|
|Flow Rates||Multiple flow rate options with user-interchangable plug-and-play flow modules to switch between nanoflow (100 nl/min - 1 µl/min) and microflow (1 - 10, or 5 -50 µl/min)|
|Flow Accuracy||SCIEX's patented Microfluidic Flow Control technology, for consistent, accurate retention times without flow-splitting|
|Software||Integrated and controlled with SCIEX Analyst software|
Better quality data for your lab is just a click away. Consult with us to see how nanoflow can help you overcome your biggest challenges.
For research use only. Not for use in diagnostic procedures.
|Switch easily from nanoflow to microflow with interchangeable flow modules
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|Shorter run times and higher throughput can be achieved using microflow instead of nanoflow. Plug & play modules enable effortless switching, and a faster autosampler supports the move from biomarker discovery to verification assays.|
|Retention time reproducibility
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For quantitative proteomics, maintaining retention time reproducibility is crucial.
The latest in SCIEX’s Microfluidic Flow Control technology, MFC Plus™, uses fast flow measurement in combination with automatic feedback to generate accurate and reproducible flow rates without flow splitting. The result is gradient reproducibility typically better than 0.35% at flow rates as low as 100 nl/min.
To achieve the best possible retention time reproducibility, an optional column oven capable of temperatures up to 60°C is available for all nanoLC 400 systems. Elevated temperatures can also be employed to decrease column pressure and improve separation efficiency.
For advanced applications like sample pre-concentration or dual-column set-up, the SCIEX nanoLC 400 autosampler accommodates one or two 10-port nano valves rated at 10,000 psi and have an extremely low port-to-port volume of < 25 nl.
The system’s control software enables users to rapidly create experiments and modify methods. Its method editing wizards and built-in diagnostic tools provide ease-of-use to help maximize uptime.
|Dimensions||22” x 26” x 9” (56 x 66 x 23 cm) (W x D x H)|
|Weight||57 pounds (26 kg)|
|Working temperature||15°C to 30°C|
|Maximum pressure||10,000 psi|
|Models Available||NanoLC 415 1 loading pump plus 1 gradient pump
NanoLC 425 1 loading pump plus 2 gradient pumps
|Flow rate ranges|
|Loading pump||1-50 μL/min|
|Gradient with nano flow module||100-1000 nL/min (up to 2 μL/min at reduced maximum pressure)|
|Gradient with low micro flow module||1-10 μL/min (up to 20 μL/min at reduced maximum pressure)|
|Gradient with high micro flow module||5-50 μL/min|
|Flow rate accuracy||< 1% @ 500 nL/min with nano flow module installed|
|Retention time precision||< 1% @ 500 nL/min with nano flow module installed and column at constant temperature (± 0.2 °C)|
|Gradient delay||< 25 nl|
|Wetted parts||stainless steel, titanium, PEEK, fused silica, FEP|
|I/O||1 x USB for instrument control each pump channel
2 x TTL in
8 x contact closure out
|Instrument control||Eksigent software for stand alone use
Eksigent software with drivers for Analyst® software (SCIEX)
|Digitizing the Proteomes From Big Tissue Biobanks||12/26/2018|
|Exploring the Sensitivity Differences for Peptide Quantification in the Low Flow Rate Regime||08/27/2018|
|Micro/Nano Columns Part List||08/21/2018|
|LC-MS-MS Solution for Determination of Nitrosamine Disinfection byproducts in Drinking Water||05/20/2018|
|Simplifying the Use of Ion Libraries during Data Processing of SWATH Acquisiton Proteomics Data||04/10/2018|
|Configuring NanoLC 425 for Nanoflow and Microflow LC-MS Workflows||12/20/2017|
|Using Multi-Omics Analysis to Study EMT Model of Prostate Cancer||06/04/2017|
|Proteome-wide Profiling of Drug Dose-Response in Prostate Cancer Cell Lines by Microflow SWATH Acquisition||06/04/2017|
|Dev of Hi-Throughput Proteomics Pipeline-SWATH Profiling Biochemical Individuality||06/04/2017|
|Impact of High-Resolution MRM Analysis on Complex Proteomes||05/02/2017|
|Achieving High Reproducibility for Quantitative Proteomics with Nanoflow LC-MS||05/01/2017|
|Assessing Impact of Extended Gradient Lengths for Microflow SWATH Acquisition||09/15/2016|
|NanoLC Systems for TripleTOF systems Test and Data Log||09/01/2016|
|Large-Scale Quantitative Measurements of Rat Brain Hippocampus Membrane Proteome in Prolonged Simulated Microgravity Model by 2D-SWATH Mass Spectrometry||06/06/2016|
|Accelerating Data Independent Acquisition with Microflow Chromatography||06/06/2016|
|Improving DIA Quantitation with microLC Using a Longer Gradient||06/06/2016|
|Host Cell Protein Analysis by Microflow-LC High Resolution SWATH-MS of Vaccine Samples Under Development||06/06/2016|
|Comprehensive Relative Quantification of the Cytochromes P450 by Micro-LC and SWATH Acquisition||06/06/2016|
|Declaration of Conformity for NanoLC 415 and NanoLC 425||06/01/2016|
|NanoLC 400-Configured for Microflow Flow Rates System Integration Test||06/01/2016|
|Industrializing Quantitative Proteomics Using Microflow LC and SWATH Acquisition||04/27/2016|
|Extending Depth of Coverage with SWATH Acquisition Using Deeper ION Libraries||12/10/2015|
|NanoLC 400 System - Replace the Piercing Needle||11/30/2015|
|M3 MicroLC System Overview - Sensitivity and Throughput for Precious Samples||11/01/2015|
|NanoLC 400 Systems Consumables and Accessories List||09/01/2015|
|Ultrafast, High Sensitivity microLC-MS/MS for Peptide Quantitation in Highly Targeted Assays||06/01/2015|
|User Guide: Ekspert nanoLC System Integration Tests||05/29/2015|
|Characterization of Intact and reduced therapeutic monoclonal antibodies using microflow size exclusion chromatography coupled with mass spectrometry||06/19/2014|
|Unlocking the Benefits of MicroLC with SCIEX and Eksigent Technology||06/17/2014|
|Ultra-Sensitive Quantitation of pg/mL Level Exenatide with Trap-and-Elute Micro-Flow LC/MS/MS||06/16/2014|
|Exploring microLC/MS/MS for Accelerating Peptide Quantitation Assays||06/15/2014|
|ekspert nanoLC 400 Systems Site Planning Guide||05/05/2014|
|Reproducible Analysis Whole Proteome Quantitation||01/06/2014|
|Reproducible Analysis for Whole Proteome Quantitation Using the Eksigent nanoLC 400||12/20/2013|
|Ekspert NanoLC 400 Systems Operator Guide||11/02/2013|
|On-line, Chip-Based Strategy for Increased Depth of Coverage in Proteomic Samples||05/03/2013|
|Achieve Low Flow Sensitivities with Micro Flow Chromatography on the QTRAP 6500 System for Targeted Quantitative Proteomics||05/01/2013|
|Brochure: Ekspert NanoLC 400||05/01/2012|
|Exploring the Sensitivity Differences for Peptide Quantification in the Low Flow Rate Regime|
|Simplifying the Use of Ion Libraries During Data Processing of SWATH Acquisition Proteomics Data|
|Microflow SWATH Acquisition for Industralized Quantitative Proteomics|
|Achieving Very High Reproducibility for Quantitative Proteomics with Nanoflow LC/MS|
|Configuring the NanoLC 425 System for Both Nanoflow and Microflow LC-MS Workflows|
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