NanoLC 400: A low flow LC-MS solution

The ideal choice for any workflow

The NanoLC 400 series enables a full range of analytical workflows that drive the identification of novel biomarkers or the quantification of small molecules and biologics to advance precision medicine research.

This front-end system consistently delivers accurate retention times, reproducible injection down to 200 nL and covers a broad range of flow rates.

NanoLC 400

The choice is yours

The NanoLC 400 Series is available in two models, with high pressure pumps that make these systems among the most accurate nanoflow models on the market. Combined with industry-leading SCIEX mass spectrometers, the NanoLC Series provides high sensitivity, robustness and ease of use to support the latest in data-independent quantitative proteomics with SWATH® Acquisition.

The NanoLC 425 is an ideal HPLC system for labs performing a broad range of proteomics analyses. The additional pump gives you the power to dive deeper into your sample – with the ability to see more using 2D workflows. With the flexibility to have pre-set nano and microflow configurations, this 2-in-1 system is ideal for labs with diverse applications that need to actively switch between flow regimes. Like the NanoLC 415, this integrated system includes an autosampler, solvent organizer, sample storage and cooling; plus a column oven – helping your lab to realize its full potential.

When your sample is limited, and sensitivity is needed choose the NanoLC 415 for direct-to-column or trap-and-elute workflows. The NanoLC 415 is a complete package, providing an autosampler, solvent organizer, sample storage and cooling giving you the confidence that you will be up and running quickly.

Meeting the needs of many workflows

With single-gradient and dual-gradient models available - workflows from simple trap and elute to complex multi-dimensional separation are possible. The NanoLC 400 Series readily fits the ever-changing needs of your lab.

When samples are precious

When you only have minimal amounts of sample, the NanoLC 400 Series can get you the high-quality identification and quantitation data you need for new biological insights.

Industrialize your workflow

When you are ready to industrialize your workflow, the NanoLC 400 Series can readily move to microflow to give you more robustness and higher throughput.

Excellent separations every time

Microfluidic FlowControl™ technology, you get enables consistent, and accurate results without flow splitting, even at high pressures.

Built for simple or complex workflows


  Key features NanoLC 400 Series
NanoLC 415 for basic direct-to-column or trap-and-elute workflows
NanoLC 425 for more advanced 2D workflows
Column Oven Compatible with the OptiFlow® Turbo V coumn oven. Column oven provides excellent retention time reproducibility by ensuring consistent column temperature
Flow Rates Multiple flow rate options with user-interchangable plug-and-play flow modules to switch between nanoflow (100 nl/min - 1 µl/min) and microflow (1 - 10, or 5 -50 µl/min)
Flow Accuracy SCIEX's innovative multifluidic flow control technology, for consistent, accurate retention times without flow-splitting
Software Integrated and controlled with SCIEX Analyst® Software