AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.

TripleTOF® 5600+ System

TripleTOF® 5600+ System

Setting the QTOF Standard for High Performance Qualitative and Quantitative Analysis on a Single Platform

The TripleTOF 5600+ System combines the best-in-class front end of SCIEX Triple Quad technology with the Accelerator TOF Analyzer, to deliver nominal mass and accurate mass benefits in a single platform.

This proven workhorse uniquely integrates comprehensive, qualitative exploration, rapid profiling, and high-resolution quantitation workflows, providing high performance and versatility for pharmaceutical development, omics research and any challenging unknown compound analysis.

Find Out More from Every Sample

  • Confidence in comprehensive coverage with complete and unbiased SWATH® Acquisition
  • Reliability and maximum uptime with stable mass accuracy for over 50 hours of continuous LC-MS operation
  • Sensitive, high-resolution system – with low limits of quantitation (LOQ) equivalent to high-performance triple quadrupoles
  • Suited for regulated environments to support 21 CFR Part 11 Compliance

Status: Available >

High Resolution Accurate Mass Workhorse

Explore the Unique TripleTOF System Technology

Downloads & Resources

RUO-MKT-18-1925-C

Overview

The TripleTOF 5600+ System is the next generation in quadrupole TOF technology. Combining the best-in-class front end of the Triple Quad 5500 System with the Accelerator TOF Analyzer, this instrument adds high resolution at the speed and sensitivity needed to give %CVs and limits of quantitation (LOQ) equivalent to those of high-end triple quadrupoles.

Now for the first time, pharmaceutical, academic, and analytical testing scientists can integrate comprehensive, qualitative exploration, rapid profiling, and high-resolution quantitation of low-abundance analytes in very complex matrices – all on a single platform.

Feature Benefit
MS/MSALL with SWATH® Acquisition Quantitative MS/MS data that rivals triple quadrupole sensitivity and precision for every component in complex samples.  And... a complete digital archive of all precursor and product ions in a sample, for unlimited retrospective interrogation without the need to re-acquire data.
21 CFR 11 Compliance Module Software tools that support digital record-keeping and electronic signatures, enabling the use of TripleTOF® Technology in a regulated environment.
Highest sensitivity with QJet Ion Guide and Accelerator TOF Analyzer Low-abundance analytes easily discovered, identified, and quantified
SmartSpeed 100Hz Acquisition The fastest, high-resolution system that generates more high-quality information from every sample, even when using fast chromatography
EasyMass Accuracy Achieves high mass-accuracy without continuous recalibration, making it the easiest-to-use system for routine accurate mass.
One-touch productivity software Increases productivity by facilitating powerful workflows to create information out of data
High resolution at up to 100 spectra per second Definitive identification at high speed even when using fast chromatography
Magnetic levitation (MagLev) turbo pumps New level of reliability for reduced cost of ownership and virtually silent running for a better lab environment.
Explore Workflow 5600 Explore Workflow thumbnail Explore complex samples in greater depth: Select up to 50 precursors per second – and generate high resolution and high mass-accuracy MS/MS data using powerful IDA (Information-Dependent Acquisition) algorithms and high-resolution, accurate-mass MS and MS/MS.
Profile Workflow
SCIEX TripleTOF™ 5600 profile workflow thumbnail		 Profile samples for quant and qual in a single run: Quantify virtually every compound in the sample with high-resolution MS quantification and fast chromatography; confirm compound identity with high-resolution, accurate-mass MS/MS; and then mine the data for the information you need.
Quantify Workflow SCIEX TripleTOF™ 5600 System quantify workflow thumbnail Quantify analytes with high-resolution MRM transitions for highest specificity and confidence: Obtain high resolution at high and low masses in the same spectrum for high-resolution, MRM-like quantification. The LOQ's and dynamic range are equivalent to high-performance triple quadrupoles. Cycle times are as low as 10 msec and generate superior peak definition, even with fast LC separations. Quantification requires little optimization, and you can choose multiple fragments per precursor from the acquired full-scan MS/MS data.

RUO-MKT-18-1925-A

Resources
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Title Date
User Guide: NanoSpray Ion Source Operator Guide (IT) 04/17/2019
User Guide: NanoSpray Ion Source Operator Guide (DE) 04/17/2019
User Guide: NanoSpray Ion Source Operator Guide (FR) 04/17/2019
User Guide: NanoSpray Ion Source Operator Guide (EN) 04/17/2019
Rapid Identification and Quantification of Novel Psychoactive Substances in Human Whole Blood Using SWATH® Acquisition 03/26/2019
Ion Source Tests, Specifications, and Data Log (EN) 03/01/2019
Ion Source Tests, Specifications, and Data Log (ZH) 03/01/2019
Ion Source Tests, Specifications, and Data Log (FR) 03/01/2019
Ion Source Tests, Specifications, and Data Log (ES) 03/01/2019
Ion Source Tests, Specifications, and Data Log (DE) 03/01/2019
Ion Source Tests, Specifications, and Data Log (JA) 03/01/2019
Ion Source Tests, Specifications, and Data Log (IT) 03/01/2019
Separation of Ephedrine and Pseudoephedrine Isomers Using SCIEX SelexION Differential Mobility Technology 02/15/2019
Digitizing the Proteomes From Big Tissue Biobanks 12/26/2018
Turbo V Ion Source Operator Guide (ES) 12/01/2018
Turbo V Ion Source Operator Guide (PL) 12/01/2018
Turbo V Ion Source Operator Guide (PT) 12/01/2018
Turbo V Ion Source Operator Guide (RU) 12/01/2018
Turbo V Ion Source Operator Guide (ZH) 12/01/2018
Turbo V Ion Source Operator Guide (JA) 12/01/2018
Turbo V Ion Source Operator Guide (IT) 12/01/2018
Turbo V Ion Source Operator Guide (DE) 12/01/2018
Turbo V Ion Source Operator Guide (FR) 12/01/2018
Turbo V Ion Source Operator Guide (EN) 12/01/2018
Resolution of Ether and Diacyl-Linked Phospholipids by Differential ION Mobility Spectrometry (DMS) 11/19/2018
Metabolomics Online Symposium Cajka 10/16/2018
Differential Mobility Spectrometry Resolves Chlorine- and Acetate- Adducts of Phosphatidylcholine (PC) 10/09/2018
Enabling Systems Biology Driven Proteome Wide Quantitation of Mycobacterium Tuberculosis using SWATH Acquisition 06/28/2018
BioPharmaView Software 3.0 Product Bulletin 05/01/2018
Simplifying the Use of Ion Libraries during Data Processing of SWATH Acquisiton Proteomics Data 04/10/2018
Triple TOF 5600+ Declaration of Conformance 03/12/2018
Multi-Omics Analysis of Human Embryonic Stem Cell Neural Differentiation 12/22/2017
User Guide: TripleTOF 5600/5600+ Instruments System User Guide 12/20/2017
User Guide: TripleTOF 5600/5600+ Instruments System User Guide (FR) 12/20/2017
User Guide: TripleTOF 5600/5600+ Instruments System User Guide (DE) 12/20/2017
User Guide: TripleTOF 5600/5600+ Instruments System User Guide (IT) 12/20/2017
User Guide: TripleTOF 5600/5600+ System Qualified Maintenance Person Guide 12/20/2017
User Guide: TripleTOF 5600/5600+ System Qualified Maintenance Person Guide (DE) 12/20/2017
User Guide: TripleTOF 5600/5600+ System Qualified Maintenance Person Guide (FR) 12/20/2017
User Guide: TripleTOF 5600/5600+ System Qualified Maintenance Person Guide (IT) 12/20/2017
User Guide: DuoSpray Ion Source for TripleTOF Systems Operator Guide (FR) 12/20/2017
User Guide: DuoSpray Ion Source for TripleTOF Systems Operator Guide (IT) 12/20/2017
Automated HIgh Throughput Trypsin Digests for HIghly Reproducible Peptide Map Analysis 12/20/2017
User Guide: TripleTOF 5600/5600+ System Qualified Maintenance Person Guide (ES) 12/20/2017
User Guide: TripleTOF 5600/5600+ Instruments System User Guide (ES) 12/20/2017
Expanding Suspect Screenig and Non-Targeted Identification 12/20/2017
Dont miss Synthetic Drug Present in Sample 12/20/2017
Improved Data Quality Using Variable Q1 Window Widths in SWATH Acquisition 12/11/2017
Ultra-sensitive Host Cell Protein Dection using CESI-MS with SWATH Acquisition 12/10/2017
Over 17K Compounds Available-X500R and Spectral Libraries 12/10/2017
Extending Metabolome Coverage-untargeted Metabolomic Workflows 12/05/2017
SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids 11/30/2017
DuoSpray Ion Source for TripleTOF Systems Operator Guide (EN) 10/10/2017
DuoSpray Ion Source for TripleTOF Systems Operator Guide (DE) 10/10/2017
TripleTOF 5600+ Site Planning Guide (EN) 10/10/2017
TripleTOF 5600+ Site Planning Guide (DE) 10/10/2017
TripleTOF 5600+ Site Planning Guide (ES) 10/10/2017
TripleTOF 5600+ Site Planning Guide (FR) 10/10/2017
TripleTOF 5600+ Site Planning Guide (IT) 10/10/2017
User Guide: SelexION and SelexION+ Technology Troubleshooting 09/30/2017
Quantification of Large Oligonucleotides using High Resolution MS/MS on the TripleTOF® 5600 System 09/15/2017
CloudConnect Auto Uploader 09/12/2017
Solutions for Biotherapeutic Characterization 08/01/2017
High Throughput, Data Independent Acquisition for Qualitative and Quantitative Shotgun Lipidomics 06/20/2017
asms2017-aKrotulski 06/04/2017
Using Multi-Omics Analysis to Study EMT Model of Prostate Cancer 06/04/2017
Proteome-wide Profiling of Drug Dose-Response in Prostate Cancer Cell Lines by Microflow SWATH Acquisition 06/04/2017
Dev of Hi-Throughput Proteomics Pipeline-SWATH Profiling Biochemical Individuality 06/04/2017
SCIEX Quantitation and Metabolism Solutions Brochure 05/26/2017
MetabolitePilot Software 2.0 05/04/2017
Impact of High-Resolution MRM Analysis on Complex Proteomes 05/02/2017
SWATH AAFS Poster 04/04/2017
Investigating Biological Variation of Liver Enzymes in Human Hepatocytes using SWATH Acquisition 02/15/2017
MarkerView Software 1.3 New Features 12/15/2016
M3 MicroLC for TripleTOF-Test and Data Log 12/10/2016
M3 MicroLC-TE for TripleTOF-Test and Data Log 12/10/2016
Analysis of Intact MUPs (Major Urinary Proteins) from Mice by CESI-MS 11/09/2016
Why Compromise-The Benefit of High-Resolution Quantitative Analysis 09/15/2016
NanoLC Systems for TripleTOF systems Test and Data Log 09/01/2016
Combination of Isobaric Tagging Reagents and Cysteinyl Peptide Enrichment for In-Depth Quantification 08/31/2016
Comprehensive Quantitative Phosphoproteomic Approach using MS/MSALL with SWATH Acquisition 08/31/2016
PCT-SWATH for Protein Quantitation-Tissue to Peptids in 4 Hours 07/01/2016
Non-Targeted Metabolic Profiling of Human Urine Samples 05/13/2016
UHPLC-ESI-QTOF-MS/MS based Lipidomics by Data-independent Acquisition 05/11/2016
The Lipidyzer- a new platform for quantitative Lipidomics 05/11/2016
CESI-MS a technique for the characterization and analysis of both intact and digested biopharmaceuticals 05/11/2016
industrializing omics-SCIEX Life Science Research towards Precision Medicine 05/01/2016
User Guide: TripleTOF Systems Electromagnetic Environment Information 04/20/2016
Understanding Complex Lipid Metabolism through Quantitative Lipidomics 03/15/2016
SCIEX Defined Major Repairs 01/10/2016
Comprehensive Characterization of Low Molecular Weight Heparins Using High Resolution Mass Spectrometry 12/15/2015
MS/MS-ALL with SWATH Acquisition 11/10/2015
Using MS/MS with SWATH Acquisition for Forensic Drugs Screening with SCIEX TripleTOF 4600/5600+ LC-MS/MS System 10/08/2015
Eksigent MicroLC 200/200 Plus Systems 10/05/2015
Automating Protein Digestion for Reproducible Proteomics 08/03/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (DE) 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (IT) 05/31/2015
User Guide: SelexION Technology for TripleTOF Systems User Guide (FR) 05/31/2015
SelexION Technology for TripleTOF Systems User Guide (ES) 05/31/2015
Investigating Relationship btwn Biological Variability and Biomarker Discovery by SWATH MS 05/21/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (DE) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (FR) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (IT) 03/31/2015
User Guide: SelexION Technology for TripleTOF Systems Site Planning Guide (ES) 03/31/2015
Spectral Libraries for Food Analysis - Antibiotics 03/05/2015
Spectral Libraries for Food Analysis - Pesticides 03/05/2015
Metabolomic Profiling of Accurate Mass LC-MS/MS Data to Identify Unexpected Environmental Pollutants 01/01/2015
Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances 01/01/2015
Optimizing Chromatography and High Resolution TOF-MS for ADC DAR Characterization 06/15/2014
Impact of novel MS/MSALL acquisition and processing techniques on forensic toxicological screening 06/15/2014
User Guide: Calibrant Delivery System Operator Guide 06/01/2014
User Guide: Calibrant Delivery System Operator Guide (DE) 06/01/2014
User Guide: Calibrant Delivery System Operator Guide (FR) 06/01/2014
User Guide: Calibrant Delivery System Operator Guide (IT) 06/01/2014
Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances 03/01/2014
Are Pork Extracts Present in My Gummy Bears? Gelatin Speciation by LC-MS/MS 01/01/2014
Rapid Characterization of Naphthenic Acids Using High Resolution Accurate Mass MS and MS/MS with SelexION™ Differential Mobility Separation 01/01/2014
Instrument Front-End Cleaning Procedure Manual 10/01/2013
Rapid and Unbiased Quantitation of Host Cell Protein Contaminants in Biologics by MSMS of All Using a TripleTOF Mass Spectrometer 09/24/2013
Drug Screening Workflows using the TripleTOF 5600 System Advantages Compared to LC MS and LC TOF 09/13/2013
Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations 07/01/2013
Advances in Accurate Mass Spectrometry with the TripleTOF 5600 System 06/13/2013
Master Your Data to Get to the Answer, View Results with Ease and Confidence Using TripleTOF Technology and MasterView Software to Identify and Quantify Food Residues 04/01/2013
Simultaneous Quantitative Peptide Mapping and Host Cell Protein Detection 01/01/2013
Master Your Data to Get to the Answer, View Results with Ease and Confidence 01/01/2013
Master Your Data to Get to the Answer, View Results with Ease and Confidence (targeted) 01/01/2013
NanoLC Ultra System for TripleTOF 5600, QTRAP 5500 and 4000 QTRAP 03/05/2012
Profiling of Botanical Samples to Identify Unknown Chemical Contaminants and the Authenticity of Natural Ingredients Using High Resolution and Accurate Mass MS/MS 03/01/2012
High Reproducibility Targeted Quantitation at Highest Multiplexing 02/01/2012
Biomarker and Omics Solutions for Metabolomics 02/01/2012
Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays 01/01/2012
Authenticity Assessment of Fruit Juices using LC-MS/MS and Metabolomic Data Processing 01/01/2012
Fast and Selective Quantification of Opiates in Urine using LDTD-TripleTOF® 5600 System 01/01/2012
ImpurityPilot Software 1.0 Beta 08/01/2011
Using PeakView Software with the XIC Manager for Screening and Identification with High Confidence based on High Resolution and Accurate Mass LC-MS/MS 04/02/2011
Targeted and Non-Targeted Screening for Drugs with High Confidence based on High Resolution and Accurate Mass LC-MS/MS 03/01/2011
Broader Coverage and Automatic Mass Calibration Using the TripleTOF® 5600 System with DuoSpray™ Ion Source 03/01/2011
MALDI Imaging of an Italian Banknote 03/01/2011
LC-MS/MS Based Strategy for the Non-Targeted Screening of an Unlimited Number of Contaminants in Food Using the SCIEX TripleTOF® 5600 System and Advanced Software Tools 03/01/2011
ProteinPilot™ Descriptive Statistics Template 03/01/2011
Brochure: Drug Discovery and Development Solutions 01/01/2011
Analysis of Polycyclic Aromatic Hydrocarbons (PAH), Alkylated Derivatives, and Photo-degradation Products in Environmental and Food Samples using LC-FLD-MS/MS with QTRAP Technology 01/01/2011
LC-MS/MS Solutions for Lipidomics - For Discovery and Targeted Lipidomics 01/01/2011
Molecular Characterization and Quantitation of Lipids with High Resolution Accurate Mass Tandem MS Techniques 01/01/2011
Targeted and Non-Targeted Screening for Drugs with High Confidence based on High Resolution and Accurate Mass LC-MS/MS 01/01/2011
Role of Sciex Mass Spectrometers in Ginseng R & D 01/01/2011
Scientist Respond to the Sciex Triple TOF 5600 System 07/19/2010
AB SCIEX Launches Groundbreaking Mass Spectrometry System 05/24/2010
Simultaneous Peptide Quantitation and Identification using High-Resolution MS: Profile Workflow on the SCIEX TripleTOF® 5600 System 03/05/2010
Breakthrough Productivity for ADME Studies with the New SCIEX TripleTOF® 5600 System 03/05/2010
Target and Non-Target Screening for Pesticide Residues in Food Samples using the SCIEX TripleTOF® 5600 System 03/05/2010
Metabolomic Profiling of Accurate Mass LC-MS/MS Data to Identify Unexpected Environmental Pollutants 02/01/2010
User Guide: NanoSpray Head and Emitter Tip Assembly Quick Start Guide
MetabolitePilot Introduction
Simplifying the Use of Ion Libraries During Data Processing of SWATH Acquisition Proteomics Data
Multi-Omics analysis of Human Embryonic Stem Cell
Software
MasterView 1.1 Software
  • Version: 1.1
  • Release Date: 12.01.14
  • File Size 560 MB
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PeakView 2.1 Software
  • Version: 2.1
  • Release Date: 01.01.14
  • File Size 537 MB
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PeakView 2.2 Software
  • Version: 2.2
  • Release Date: 01.01.14
  • File Size 530 MB
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PeakView 2.0 Software
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  • Release Date: 01.01.14
  • File Size 569 MB
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MasterView 1.0 Software
  • Version: 1.0
  • Release Date: 12.01.13
  • File Size 1.5 GB
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ImpurityPilot Software 1.0 Beta
  • Version: 1.0
  • Release Date: 08.01.11
  • File Size 541 MB
ImpurityPilot Software 1.0 Beta Release Notes
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TT5600_5-col2

Dig Deeper in Your Sample

With optimized ion production, focusing, reflection and detection, the TripleTOF 5600+ System will help you dig deeper into complex samples.

IonDrive Turbo V Source

The new design of the legendary Turbo V source increases ion production by using enhanced gas flow dynamics and optimized heater configuration to improve reliability, reproducibility, and robustness at higher flow rates.

SWATH® Acquisition

QJet® Ion Guide

Designed for robustness, the QJet Ion guide provides large capture radius for efficient transfer of ions from source to quadrupole optics.

SelexION Technology

High Frequency LINAC® Collision Cell

High drive frequency collision cell provides better ion transmission, higher duty cycle, and improved resolution by focusing ions prior to entering TOF.

SWATH® Acquisition

Accelerator TOF™ Analyzer

Time-of-flight analyzer uses a 2-stage mirror and 15 kV acceleration to improve resolution and efficiency. Get the speed and sensitivity required for triple quadrupole like quantitation, but with high resolution and mass accuracy for qualitative applications.

SelexION Technology

Detection System

4-channel MCP detector with TDC processing technology provide more than 4-orders of linear dynamic range while improving robustness and lifetime at high ion currents.

TT5600_Image_Callout
1. Enhanced Selectivity with Differential Ion Mobility

Get more out of every sample with an additional dimension of separation with SelexION® Differential Ion Mobility Technology.

2. Robust Spray with the DuoSpray™ Turbo V Source

Efficient ion production while limiting contamination for maximized robustness, reliability and uptime.

TripleTOF 5600+ System
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3. Comprehensive Coverage with Data-Independent SWATH® Acquisition

Variable window capabilities allow for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples.

4. Maximize Flexibility with Front End Separations

Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow, and capillary electrophoresis spray ionization (CESI).

TT5600_video_comp
TT5600

Watch Video

Powerful. Productive. Versatile.

Take a tour of the 5600 that will power your workflows.

Video Length 03:44

TT5600_Table
Key Features
Mass Range TOF 40 kDa
Mass Selection Range Q1 5 – 1250 m/z
TOF-MS Mass Resolution and Acquisition Speed ≥35,000 (FWHM) at 956 m/z, using 50 ms accumulation time
TOF-MS/MS Mass Resolution and Acquisition Speed ≥25,000 (FWHM) at 195 m/z, and ≥18,000 (FWHM) at 86 m/z, using 50 ms accumulation time
Detector Type 4-channel MCP detector with TDC signal processing for extended dynamic range
Flexible Data Acquisition Workflows TOF-MS
Information dependent acquisition (IDA) MS/MS
Data Independent SWATH® Acquisition with fixed or variable Q1 isolation windows
Targeted MRMHR workflow
Ionization Sources IonDrive™ Turbo V, DuoSpray™ source, each with TurboIonSpray® or Atmospheric-pressure chemical ionization (APCI) probes available; NanoSpray®III source, Digital PicoView NanoSource
Orthogonal Front End Separation Compatibility Compatible with advanced front-end separation strategies including high-flow, microflow and nanoflow chromatography, capillary electrophoresis spray ionization (CESI), or using SelexION® differential ion mobility
Variable Window SWATH® Acquisition Data-Independent SWATH® Acquisition with variable window capabilities, allows for a large number of Q1 isolation windows at user controlled widths, up to 200 per cycle to achieve better specificity, even in complex samples. Read More
Data File Portability Condensed data file sizes simplify and streamline data storage and portability for sharing with colleagues, without losses in data quality, completeness or security
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AB Sciex is doing business as SCIEX. © 2010-2019 AB Sciex. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Beckman Coulter® is being used under license. Product(s) may not be available in all countries. For information on availability, please contact your local representative. For research use only. Not for use in diagnostic procedures.