Proper controls are required for reliable, reproducible proteomics experiments to ensure consistent instrument performance across experiments, studies, and users. As workflows become industrialized and move to higher throughput, the requirement for good calibration and performance standards becomes even higher.
In order to assess system suitability of the LC-MS systems, four performance suitability tests can be performed. The suitability tests have been designed for both microflow and nanoflow regimes.
- PepCalMix Infusion Test – Used to check MS performance and optimize the source positioning. Requires PeakView® Software.
- PepCalMix LC-MS System Suitability Test – Used to check the LC-MS system is in basic working order, and used for routine mass calibration. Requires PeakView® Software.
- Cell Lysate SWATH® Acquisition QC Test – Used to assess the performance of the SWATH® Acquisition. Requires SWATH® Acquisition MicroApp 2.0.1 in PeakView® Software and the SWATH® Replicate Analysis template.
- Cell Lysate IDA QC Test – Used to assess protein identification performance. Requires ProteinPilot™ Software.
You can download free and trial versions of the required software packages from our Software Downloads page.
Summary table of available performance kits
|SWATH® Acquisition Performance Kit||PepCalMix
K562 Protein Extract Digest
Manual for use
|5045757||Cell Lysate SWATH® QC Test
Cell Lysate IDA QC Test
|MS Synthetic Peptide Calibration Kit||PepCalMix
Manual for use
|5045759||PepCalMix Infusion Test
PepCalMix LC-MS System