SWATH Acquisition Performance Kit

Acquire your proteomics data with confidence using our quality control and performance kits

For research use only. Not for use in diagnostic procedures.

SCIEX is pleased to offer the SWATH Acquisition Performance Kit to assess performance of your TripleTOF® LC-MS system. The kit contains a pooled mixture of 20 synthetic peptides (PepCalMix) which provide coverage over a wide range of masses and retention times, as well as a complex digested lysate for assessing protein quantitation performance using SWATH Acquisition. The synthetic peptides are also available for purchase separately for routine mass calibration or use as retention time calibration peptides. A detailed guide to running the tests is included for both microflow and nanoflow LC rates, along with an easy-to-use template for processing the data

  • Quickly assess performance of your SWATH Acquisition experiments
  • Ensure consistent performance of your instrument for maximum uptime
  • Easily transition your methods between libraries and flow rates

Status: Available Item status information

 

Overview

Proper controls are required for reliable, reproducible proteomics experiments to ensure consistent instrument performance across experiments, studies, and users. As workflows become industrialized and move to higher throughput, the requirement for good calibration and performance standards becomes even higher.

In order to assess system suitability of the LC-MS systems, four performance suitability tests can be performed. The suitability tests have been designed for both microflow and nanoflow regimes.

  • PepCalMix Infusion Test – Used to check MS performance and optimize the source positioning. Requires PeakView® Software.
  • PepCalMix LC-MS System Suitability Test – Used to check the LC-MS system is in basic working order, and used for routine mass calibration. Requires PeakView® Software.
  • Cell Lysate SWATH® Acquisition QC Test – Used to assess the performance of the SWATH® Acquisition. Requires SWATH® Acquisition MicroApp 2.0.1 in PeakView® Software and the SWATH® Replicate Analysis template.
  • Cell Lysate IDA QC Test – Used to assess protein identification performance. Requires ProteinPilot™ Software.

You can download free and trial versions of the required software packages from our Software Downloads page.

Kits

 

   

Kit

Components

P/N Use
SWATH® Acquisition Performance Kit PepCalMix
K562 Protein Extract Digest
Manual for use
5045757 Cell Lysate SWATH® QC Test
Cell Lysate IDA QC Test
MS Synthetic Peptide Calibration Kit PepCalMix
Manual for use
5045759 PepCalMix Infusion Test
PepCalMix LC-MS System
Suitability Test

Resources

Filter by Type:

SWATH® Acquisition Performance Kit Protocol

Ultra-sensitive host cell protein detection using CESI-MS with SWATH® Acquisition

No Slide Title

Extending the Depth of Coverage in SWATH® Acquisition with Deeper Ion Libraries

Accelerating Lipid Profiling Acquisition Strategies with Differential Mobility Spectrometry and SWATH® Data Collection

The use of smaller Q1 isolation windows improves reproducibility in SWATH® based protein quantification even at higher spectral acquisition rate

SWATH® acquisition enables precise label-free quantification on proteome-scale

No Slide Title

No Slide Title

SWATH –Data Independent Acquisition Strategies for Ultimate Confidence in Peptide Mapping and Post Translational Modifications Quantitation for Biotherapeutics Characterization

Multi-Omics Analysis of hESC Neural Differentiation

Improved Data Quality Using Variable Q1 Window Widths in SWATH Acquisition

Investigating Biological Variation of Liver Enzymes

Comprehensive Quantification with Qualitative Confirmation using the TripleTOF 5600 System

Assessing Impact of Extended Gradient Lengths for Microflow SWATH Acquisition

TripleTOF Environmental Profiling_AB SCIEX_0460210-02.docx

SWATH� Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids

Comprehensive Quantification with Qualitative Confirmation using the TripleTOF 5600 System

MSMSall with SWATH Acquisition

SWATH® Acquisition Improves Metabolite Coverage over Traditional Data Dependent Techniques for Untargeted Metabolomics

Proteome Wide Quantitation of Mycobacterium Tuberculosis

Microflow SWATH Acquisition for Quantitative Proteomics

SWATH® Acquisition Allows a Deeper Level of Comprehensive Metabolite Quantitation