iMethod™ Application for THC-COOH for Cliquid® Software (Partner Contributed)

For research use only. Not for use in diagnostic procedures.

Cannabis use is detected by identifying the presence of the major psychoactive constituent of marijuana, Δ9-tetrahydrocannabinol (THC),  and its metabolites in biological fluids (e.g. urine). The major metabolite found in urine is 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH), which exists in both the free and glucuronide form.

iMethod Application for THC-COOH for Cliquid Software Partner Contributed


Samples are prepared by the dilution and hydrolysis of urine to remove any glucuronidates followed by automated, online clean-up using a Spark Holland HySphere C8EC-SE sorbent cartridge upon injection in a Symbiosis PICO system. More in-depth sample preparation and instrument parameter information is included with the method, as well as the required analytical columns. Solvents, standards, and any supplies required for sample preparation are not included.

For system requirements and example results, read the flyer: iMethod™ Application for THC-COOH for Cliquid® Software.

Reduce development time

Activity Using an iMethod Application Traditional Method Development
Complete Test Protocol Documentation Included Weeks–Months
Sample Prep Techniques Included, with examples and references Weeks–Months
Optimized MS/MS Acquisition Parameters Included Weeks–Months
LC Instrument Parameters with Retention Times Included Days–Weeks
Integration (Quantitation) Parameters Included Days
Reporting Templates Included Days
LC/MS/MS Library Spectra May be included. Stand-alone libraries are also available. Weeks–Months
Staff Training – Software Simple Cliquid® Software interface, no additional training Traditional MS Software and review procedures – Days
Method Validation Weeks. Validation templates and support available Weeks–Months
Complete Test Development and Deployment Weeks 6–12 months