vMethod applications from SCIEX are designed to help labs implement challenging new applications with ease and be ready for validation with minimal method development

The updated vMethod application for forensic toxicology screening adds 130 novel psychoactive substances (NPS) to the original screening method for 664 drugs of abuse. It makes full use of the unique data independent acquisition (DIA) capabilities of SWATH DIA with confirmation through full-spectral MS/MS library entries. A complete standard operating procedure (SOP) provides details on:

• Sample preparation for human whole blood and urine
• Comprehensive LC-MS/MS methods that employ either SWATH DIA or data dependent acquisition (DDA) for analysis
• Reliable data processing for quantification and confirmation of over 790 analytes with meaningful reporting tools

Working closely with expert collaborators around the world, SCIEX has developed a robust and reliable workflow for the screening of drugs of abuse and their metabolites in blood and urine. It allows laboratory personell  in forensic laboratories to quickly and easily get up-and-running, processing samples and getting results-, with no need for extensive method development or in-depth training. 

Users new to accurate mass spectrometry will be able to easily validate the method and start routine analyses with confident reporting of results thanks to the ease of use of the latest SCIEX OS software.

Learn how to use this vMethod application effectively with our content pack, which includes:

• Information on the original vMethod application for 664 compounds
• The technical notes associated with both versions of the vMethod application
• A video in which Pierre Negri, Forensic Market Manager at SCIEX, explains the updated version of the vMethod application 
• Links to instructional resources that detail the technologies and workflows, along with a list of the compounds included in the Forensic HR-MS/MS Spectral Library