ADC eBook

Antibody-drug conjugates (ADCs) are among the most analytically challenging biotherapeutics, as they combine an antibody, linker, and cytotoxic payload into a single molecule. This creates interconnected sources of heterogeneity, including DAR, charge variants, PTMs, structural modifications, and conjugation sites that must be characterized, understood, and monitored together.

Download the eBook to explore how integrated CE and LC-MS workflows can help biopharma teams move beyond individual measurements to a more complete understanding of ADC structure, quality, and consistency across the development lifecycle.

In this brochure, you’ll discover analytical strategies to:

Quantify ADC components to support stability, safety, and efficacy assessment
Resolve charge variants and structural isoforms with greater confidence
Connect charge separation, mass detection, and peptide-level characterization for more interpretable insight
Use high-resolution LC-MS, native SEC-MS, and EAD-based workflows to support DAR analysis, PTM identification, payload localization, and structural interpretation
Apply quantitative LC-MS workflows for antibody, free payload, conjugated antibody, low-level variants, and impurities

How SCIEX analytical solutions support ADC analysis

ADC characterization challenges are not just about measurement. They are about connecting multiple attributes into a single, interpretable understanding of structure. To address these challenges, ADC analytics is shifting toward orthogonal strategies that combine:

Intact mass analysis for DAR determination
Intact analysis for charge heterogeneity, PTM identification, and DAR
Subunit and peptide-level characterization
Advanced fragmentation for structural localization
Quantitative workflows for attribute tracking

These approaches allow scientists to identify molecular species, localize modifications, quantify attribute changes, and correlate observations across datasets.

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