iMethod™ Application for Benzodiazepines Version 1.2 for Cliquid® Software

For research use only. Not for use in diagnostic procedures.

The SCIEX iMethod Application for benzodiazepines enables you to be up and running quickly with preconfigured settings and report templates specific for the quantification of 30 benzodiazepines and non-benzodiazepine hypnotics (zaleplon, zolpidem, zopiclone) in plasma, blood, or urine.

Included with this method are: a Restek 5µm PFP Propyl pre-column (60 Å, 10 x 2.1 mm) and Restek 5µm PFP Propyl analytical column (60 Å, 50 x 2.1 mm).

To learn more, please view the flyer: A Rapid iMethod™ Application for Benzodiazepines in Urine



Status: Available Item status information

iMethod Application for Benzodiazepines Version 1.2 for Cliquid Software



Just download a method, add your sample, and you're ready to go

iMethod Applications for forensic toxicology provide instant LC-MS/MS methods, enabling you to generate accurate and precise results faster. Simply download a lab-proven method from the web or a CD into Cliquid Software and run your sample. All the instrument parameters are downloaded upon installation. Everything you need to start running a test immediately is included: sample preparation protocols, optimized MS and LC parameters, integration parameters, reporting templates, and documentation.

Quantification of 30 benzodiazepines in plasma, blood, or urine

Benzodiazepines and other non-benzodiazepine hypnotics, such as zaleplon, zolpidem, and zopiclone are widely prescribed psychoactive drugs for the treatment of anxiety and sleep disorders. Frequently, these substances lead to dependence and abuse, and some can affect judgment and behavior. As a result, these compounds are of great interest to forensic and clinical toxicology laboratories. The iMethod™ Application quantifies benzodiazepines in plasma, blood, or urine. Example sample preparation procedures are provided, based upon the sample matrix type and degree of clean-up required.  Sample preparations range from a simple extraction and dilution for urine to liquid-liquid extraction for blood and plasma to solid phase extraction for plasma and urine. Diazepam-d5 and doxepin-d3 are used as internal standards.

The iMethod Application for benzodiazepines is configured for use on the SCIEX Technologies 3200 and 4000 Series instruments.

The list of available iMethod™ Applications located in the iMethod Store is continually updated with the newest user-contributed and SCIEX lab-proven methods, so you can take immediate advantage of the latest advances. All iMethod™ Applications are available for individual purchase or as part of one of our complete starter packs for forensic toxicology.

Reduce development time

ActivityUsing an iMethod ApplicationTraditional Method Development
Complete Test Protocol DocumentationIncludedWeeks–Months
Sample Prep TechniquesIncluded, with examples and referencesWeeks–Months
Optimized MS/MS Acquisition ParametersIncludedWeeks–Months
LC Instrument Parameters with Retention TimesIncludedDays–Weeks
Integration (Quantitation) ParametersIncludedDays
Reporting TemplatesIncludedDays
LC/MS/MS Library SpectraMay be included. Stand-alone libraries are also available.Weeks–Months
Staff Training – SoftwareSimple Cliquid® Software interface, no additional trainingTraditional MS Software and review procedures – Days
Method ValidationWeeks. Validation templates and support availableWeeks–Months
Complete Test Development and DeploymentWeeks6–12 months