For research use only. Not for use in diagnostic procedures.

This iMethod Application quantifies 22 acid herbicides in water using a Spark Holland Symbiosis PICO system (integrated online solid phase extraction (SPE) and HPLC system) with a Mistral column oven and SCIEX API 4000 or 4000 QTRAPLC/MS/MS System.

Samples are prepared by filtration and pH adjustment followed by online SPE sample cleanup and concentration using the capabilities of the Spark Holland PICO system in conjunction with Spark Holland HySphere SPE C18 HD sorbent cartridges. More in-depth sample preparation and instrument parameter information is included with the method. Solvents, standards, and any supplies required for sample preparation are not included.

The mobile phase consists of acetonitrile/methanol/water/ammonium acetate mixture with separation on a Waters Atlantis dC18 3.5 μm HPLC column (2.1 x 50 mm).

To view detailed preparation conditions and example results, please view the flyer: A Rapid iMethod™ Application for Herbicides in Water.