iMethod™ Application for Herbicides in Water (Partner Contributed)

For research use only. Not for use in diagnostic procedures.

This iMethod Application quantifies 22 acid herbicides in water using a Spark Holland Symbiosis PICO system (integrated online solid phase extraction (SPE) and HPLC system) with a Mistral column oven and SCIEX API 4000 or 4000 QTRAPLC/MS/MS System.

Samples are prepared by filtration and pH adjustment followed by online SPE sample cleanup and concentration using the capabilities of the Spark Holland PICO system in conjunction with Spark Holland HySphere SPE C18 HD sorbent cartridges. More in-depth sample preparation and instrument parameter information is included with the method. Solvents, standards, and any supplies required for sample preparation are not included.

The mobile phase consists of acetonitrile/methanol/water/ammonium acetate mixture with separation on a Waters Atlantis dC18 3.5 μm HPLC column (2.1 x 50 mm).

To view detailed preparation conditions and example results, please view the flyer: A Rapid iMethod™ Application for Herbicides in Water.

iMethod Application for Herbicides in Water Partner Contributed


Reduce development time

ActivityUsing an iMethod ApplicationTraditional Method Development
Complete Test Protocol DocumentationIncludedWeeks–Months
Sample Prep TechniquesIncluded, with examples and referencesWeeks–Months
Optimized MS/MS Acquisition ParametersIncludedWeeks–Months
LC Instrument Parameters with Retention TimesIncludedDays–Weeks
Integration (Quantitation) ParametersIncludedDays
Reporting TemplatesIncludedDays
LC/MS/MS Library SpectraMay be included. Stand-alone libraries are also available.Weeks–Months
Staff Training – SoftwareSimple Cliquid® Software interface, no additional trainingTraditional MS Software and review procedures – Days
Method ValidationWeeks. Validation templates and support availableWeeks–Months
Complete Test Development and DeploymentWeeks6–12 months