Enabling new levels of quantification

Using the SCIEX Triple Quad 7500 system, powered by SCIEX OS software 

Abstract

With the same ruggedness and robustness of previous generations, the SCIEX Triple Quad 7500 system with D Jet ion guide and integrated E Lens probe combine to deliver significant sensitivity gains over previous generation instruments; average peak area gains of 7x and signal to noise gains of 2.5 to 3x.

RUO-MKT-02-11886-A_0

Introduction

The ability to achieve high levels of sensitivity while retaining quantitative performance is key to achieving robust analytical results. The growing number of demanding workflows requiring reliable quantification of more analytes per assay, in increasingly complex matrices, highlights the need for a sensitive MS instrument capable of providing the high level of performance that can meet these analytical challenges. The SCIEX Triple Quad 7500 system is enabling new levels of quantification across a large suite of sample types and workflows. The greater sensitivity is achieved through the introduction of hardware features that enable significant gains in the generation, capture and transmission of ions. 

Improvement in the efficiency of ion capture and transmission is because of the D Jet ion guide, which achieves greater sensitivity by sampling more ions, and without sacrificing robustness. Improved ion generation and sampling is accomplished through better desolvation, achieved by the addition of the E Lens probe that delivers more sensitivity in ESI by increasing the field strength experienced by the ESI droplets. The versatility of the OptiFlow Pro ion source provides the ability to analyze a wide range of compounds by seamlessly switching between ESI and APCI mode. In addition, the modular architecture of the source enables users to change flow rates by simply changing probes and electrodes in the source. This improved versatility enables users to achieve the best sensitivity under all conditions, future proofing labs for future innovations across many different applications.

Integrated into the SCIEX 7500 system, these technology features result in average peak area gains, across many different types of analytes, of approximately 7-fold (Figure 1), providing significant assay sensitivity gains across many applications.1,2,3   

Figure 1. Sensitivity gains for SCIEX 7500 system over QTRAP 6500+ system. Large numbers of analytes were run in various matrices on both systems and the peak areas and S/N gains were measured. (Left) Example data from propranolol in rat plasma, area gain of 9x with S/N gain of 3x. (Right) Summary of comparison of 1244 MRMs in positive and negative mode across 10 studies (pesticides, drugs, peptides), average peak area gain across the compounds was 7x.

Key innovations in the SCIEX Triple Quad 7500 system

  • D Jet ion guide—improved ion capture and transmission, combined with an orifice with increased sampling area for increased ion sampling, resulting in greater sensitivity
  • E Lens probe —improved ion generation through more energetic ESI droplet desolvation and more efficient ion collection
  • OptiFlow Pro ion source—designed for robustness and ruggedness with modular architecture to future proof the lab
  • QTRAP system upgradeable4—triple quadrupole functionality for quantification, plus full scan linear ion trap MS/MS for confirmation and MRM3 for selectivity5
  • Detection system with high energy dynode and pulse counting detector—fast polarity switching (5 msec) and up to 6 orders of magnitude across the linear dynamic range
  • SCIEX OS software—designed for quick, intuitive and streamlined data acquisition and data processing, all on one single platform6