iMethod™ Application for Screening Veterinary Drugs Version 1.4 Cliquid® Software

For research use only. Not for use in diagnostic procedures.

Get the all-inclusive veterinary antibiotics MS/MS spectral library

The SCIEX iMethod™ Veterinary Antibiotics LC-MS/MS Library Version 1.1 includes MS/MS spectra in positive and negative ion mode (where appropriate) at three collision energies for over 16 relevant antibiotics.  The collision energy spread (CES) as well as the corresponding multiple reaction monitoring (MRM) catalogue are provided, with up to three transitions per compound. Incorporate this library into your current methods or use with the iMethod™ Application for Veterinary Antibiotics Screening Version 1.1.

To learn more, please view the flyer: A Rapid iMethod™ Application for Veterinary Antibiotic LC-MS/MS Library Version 1.1 for Cliquid® Software

iMethod Application for Screening Veterinary Drugs Version 1.4 for Cliquid Software

Overview

Just download a method, add your sample, and you're ready to go

SCIEX iMethod Veterinary Antibiotics LC-MS/MS Library Version 1.1 contains information on 139 common antibiotics and their metabolites required to be monitored in meat and other food products. The MRM catalogue contains up to three transitions per compound. Each compound in the library has individual spectra acquired using three distinct collision energies (20 eV, 35 eV, 50 eV), as well as a single, aggregate spectra of all three collision energies. If compounds ionize in both polarities, spectra for both are also included, bringing the total possible spectra per compound to eight.

Use stand-alone to create custom screening and quantification methods

The MRM catalogue can be used to build methods without the need to reinfuse standards and optimize MRM transitions for a given compound. Screening and quantitation methods can be created for use with an MRM-triggered enhanced product ion (EPI) workflow and a QTRAP System; additionally, traditional quantitation can be completed using the response ratio for two or more transitions for compound confirmation. The latter approach can be performed on either an API Triple Quad System or a QTRAP Series instrument.  Simply select the compounds of interest as well as the number of transitions to be monitored from the MRM catalogue. Once selected, the Cliquid Software automatically creates the acquisition and processing methods.

Please note that this library is continually being expanded to include additional compounds. This library is verified for use on SCIEX 3200 QTRAP, 4000 QTRAP, and QTRAP 5500 LC-MS/MS Systems. 

Reduce development time

Activity Using an iMethod Application Traditional Method Development
Complete Test Protocol Documentation Included Weeks–Months
Sample Prep Techniques Included, with examples and references Weeks–Months
Optimized MS/MS Acquisition Parameters Included Weeks–Months
LC Instrument Parameters with Retention Times Included Days–Weeks
Integration (Quantitation) Parameters Included Days
Reporting Templates Included Days
LC-MS/MS Library Spectra May be included. Stand-alone libraries are also available. Weeks–Months
Staff Training – Software Simple Cliquid® Software interface, no additional training Traditional MS Software and review procedures – Days
Method Validation Weeks. Validation templates and support available Weeks–Months
Complete Test Development and Deployment Weeks 6–12 months