Development and implementation of hybrid LBA-LC-MS/MS assays for quantitation of biotherapeutics in plasma.
Training a diverse team of scientists in protein quantification
Developing a selective and highly sensitive MS/MS assay for a biotherapeutic target in plasma
Choosing the best signature peptides for quantitation
Optimization of sample enrichment and chromatographic conditions
SCIEX Biologics Quant Platform
Immunoaffinity enrichment sample prep kit with lab tested protocols
High sensitivity LC- MS/MS hardware
Powerful software for peptide MRM optimization
Scientific training and technical support from SCIEX experts
Workflow training from SCIEX for entire team to accelerate method development
Method developed for trastuzumab quantitation using three different signature peptides
Utilized immunoaffinity enrichment as a hybrid LBA-LC/MS/MS approach
Demonstrated acceptable calibration and QC for immunocaptured samples
Completed partial validation of trastuzumab biologic in rat plasma
Continued work on full validation of trastuzumab in human plasma using the hybrid LBA-LC/MS/MS approach
“The 6500 instrument and SCIEX support allows the lab to handle the analysis of a wide range of samples, including peptides, proteins and complex biological molecules, and is a natural progression of our capabilities, to serve the growing market for large molecule analysis. ”
Type of Organization
Contract research organization (CRO) Specializing in protein chemistry, mass spectrometry, and bioinformatics.
Quickly and efficiently move from small molecule to large molecule bioanalysis by LC-MS/MS. Grow SGS projects by supporting biologics bioanalysis studies.
QTRAP 6500 MS System
BioBA Sample Prep Kit
Answers for Science. Knowledge for Life.™
RUO-MKT-20-6470-A For Research Use Only. Not for use in diagnostic procedures.