SGS Belgium
Project Leaders, SGS Belgium
Project Overview
Development and implementation of hybrid LBA-LC-MS/MS assays for quantitation of biotherapeutics in plasma.
The Challenges
- Training a diverse team of scientists in protein quantification
- Developing a selective and highly sensitive MS/MS assay for a biotherapeutic target in plasma
- Choosing the best signature peptides for quantitation
- Optimization of sample enrichment and chromatographic conditions
The Solution
SCIEX Biologics Quant Platform
- Immunoaffinity enrichment sample prep kit with lab tested protocols
- High sensitivity LC- MS/MS hardware
- Powerful software for peptide MRM optimization
- Scientific training and technical support from SCIEX experts
The Outcomes
- Workflow training from SCIEX for entire team to accelerate method development
- Method developed for trastuzumab quantitation using three different signature peptides
- Utilized immunoaffinity enrichment as a hybrid LBA-LC/MS/MS approach
- Demonstrated acceptable calibration and QC for immunocaptured samples
- Completed partial validation of trastuzumab biologic in rat plasma
- Continued work on full validation of trastuzumab in human plasma using the hybrid LBA-LC/MS/MS approach
Type of Organization
Contract research organization (CRO), specializing in protein chemistry, mass spectrometry, and bioinformatics.
Goals
Quickly and efficiently move from small molecule to large molecule bioanalysis by LC-MS/MS. Grow SGS projects by supporting biologics bioanalysis studies.
SCIEX products
- QTRAP 6500 MS System
- DiscoveryQuant™ Software
- MultiQuant™ Software