Integrated mass spectrometry solutions built to streamline your vitamin analysis
Vitamin analysis for clinical research
Why LC-MS/MS is the leading choice for vitamin quantitation
Vitamins are organic compounds that play an essential role in cell function, growth, and development. They are instrumental in maintaining health and supporting various physiological functions. Assessing vitamin concentrations in the body is vital to identify potential vitamin deficiencies that can lead to a number of health disorders. Accurate quantification of low levels of vitamins in complex biological matrices is critical for clinical researchers investigating potential biomarkers of disease. However, these low concentrations make it challenging to develop highly sensitive and specific analytical methods to accurately and confidently measure these compounds.
SCIEX provides innovative LC-MS/MS solutions for vitamin analysis. Our robust portfolio of instruments offers the required levels of sensitivity and specificity to accurately quantify multiple vitamins and their metabolites, providing a comprehensive picture of vitamin status compared to traditional analytical methods such as immunoassays. Learn how our mass spectrometry solutions for clinical research can help your laboratory more accurately quantify vitamin levels in complex biological matrices.
LC-MS/MS analysis of essential vitamins in biological matrices
Vitamin A and E analysis in human serum
Workflow
Accurate measurement of vitamins A and E in human blood and serum requires exceptional sensitivity, precision, and reproducibility across a broad concentration range. Overcome analytical challenges with optimized mass spectrometry conditions and advanced solutions designed for reliability. Streamline your workflow with intuitive, user-friendly software to save time and deliver faster results.
Sensitive analysis of vitamin A and E in human serum
Key benefits:
- Superior sensitivity to acheive sub- to low-umol/L detection limits in human serum
- Excellent linearity across the calibration set
QTRAP 6500+ system
The QTRAP 6500+ system offers revolutionary sensitivity, speed, and performance for your most challenging methods.
Learn more
Quantitative analysis of vitamin A and E in human serum
Key benefits:
- Reliable and robust performance for routine analysis
- Good quantitative performance for accuracy and precision in serum-matrix calibrators
- 5.5 min runtime for high-throughput analysis
QTRAP 4500 system
The QTRAP 4500 system offers reliable quantitation with 100x more full-scan sensitivity over basic triple quadrupoles in the same class.
Learn more
Featured resources
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Technical note
Sensitive quantitation of vitamin A and E in human serum
This technical note describes a rapid protein precipitation sample preparation procedure and a robust and sensitive LC-MS/MS method using the QTRAP 6500+ system for the quantitation of vitamin A and E in human serum. Excellent linearity was observed across clinically relevant concentrations. Low-µmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 53:1 for vitamin A at 0.41 µmol/L and 753:1 for vitamin E at 5.04 µmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, demonstrating the quantitative performance of the assay.
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Technical note
Quantitative analysis of vitamin A and E in human serum
This technical note demonstrates the sensitive and accurate quantitation of vitamins A and E in human serum using a rapid protein precipitation sample preparation procedure with analysis using the SCIEX QTRAP 4500 system. Excellent linearity was observed across clinically relevant concentrations. Low-µmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 72:1 for vitamin A at 0.76 µmol/L and 105:1 for vitamin E at 6.76 µmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, highlighting the assay's strong quantitative performance.
Vitamin B1 and B6 analysis in human blood
Workflow
Ensuring the accurate measurement of vitamin B1 and B6 in human blood demands high sensitivity, precision, and reproducibility across a wide range of concentrations. Move past analytical challenges for vitamin B1 and B6 with optimized chromatographic separation and mass spectrometry conditions. Save time with intuitive software and get your data faster.
Sensitive analysis of vitamin B1 and B6 in human blood
Key benefits:
- Simple, fast protein precipitation sample preparation
- Superior sensitivity demonstrated by low nmol/L detection in whole blood-matrix calibrators
- Excellent accuracy and precision in the lowest calibration standard for accuracy and precision
- Good linearity across the calibration range
QTRAP 6500+ system
The QTRAP 6500+ system offers revolutionary sensitivity, speed, and performance for your most challenging methods.
Learn more
Analysis of vitamin B1 and B6 in human blood
Key benefits:
- Reliable and robust performance for routine analysis
- Good accuracy and precision in lowest matrix calibrator demonstrating strong quantitative performance
- 5 min runtime for high-throughput analysis
QTRAP 4500 system
The QTRAP 4500 system offers reliable quantitation with 100x more full-scan sensitivity over basic triple quadrupoles in the same class.
Learn more
Featured resources
-
Technical note
Sensitive quantitation of vitamin B1 and B6 in human whole blood
In this technical note, the combination of a rapid sample preparation procedure consisting of a protein precipitation with a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled accurate quantitation of vitamin B1 and B6 extracted from human whole blood. Excellent linearity was observed across clinically relevant concentrations. Low-nmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 769:1 for vitamin B1 at 39 nmol/L and 499:1 for vitamin B6 at 21 nmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, demonstrating the quantitative performance of the assay.
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Technical note
Quantitative analysis of vitamin B1 and B6 in human whole blood
This technical note describes a rapid protein precipitation sample preparation procedure and a robust LC-MS/MS method for the quantitation of vitamins B1 and B6 from human whole blood. Low-nmol/L level sensitivity was shown at the lowest calibrator with signal-to-noise ratios (S/N) of 260:1 for vitamin B1 at 39.0 nmol/L and 45:1 for vitamin B6 at 23.0 nmol/L. The method demonstrated excellent linearity across clinically relevant concentrations as well as excellent precision and accuracy at low-level concentrations, highlighting the assay's quantitative performance.
1,25-Dihydroxyvitamin D2 and D3 analysis in human matrix
Workflow
Accurate quantitation of 1,25-dihydroxyvitamin D2 and D3 in human matrices requires high sensitivity, precision, and reproducibility across diverse concentration ranges. Address analytical challenges for the analysis of 1,25-dihydroxyvitamin D2 and D3 with optimized chromatographic separation and mass spectrometry conditions. Enhance efficiency with user-friendly software, ensuring faster data acquisition.
Sensitive quantitative analysis of 1,25-dihydroxyvitamin D2 and D3 in human serum
Key benefits:
- Excellent quantitative performance in the lowest calibrator (5 pg/mL) demonstrated by <5%CV precision
- Good linearity across the 5-200 pg/mL calibration set
QTRAP 6500+ system
The QTRAP 6500+ system offers revolutionary sensitivity, speed, and performance for your most challenging methods.
Learn more
25-hydroxyvitamin D2 & D3 in human serum
Workflow
Reliable measurement of 25‑hydroxyvitamin D3 and related metabolites in human matrices requires high specificity, robustness, and throughput. Overcome analytical complexity with optimized LC‑MS/MS methods. Enable consistent, high‑throughput vitamin D quantitation using dependable workflows and user‑friendly software built for clinical research analysis.
Quantitative analysis of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3, and 25-hydroxyvitamin D2 in human serum
Key benefits:
- Chromatographic separation of 25-OH-vitamin D3, 3-epi-25-OH-vitamin D3, and 25-OH-vitamin D2 within the 6 minute runtime
- Excellent accuracy in the lowest matrix-calibration standard (1 ng/mL)
- Good linearity across the 1-100 ng/mL calibration range
QTRAP 4500 system
The QTRAP 4500 system offers reliable quantitation with 100x more full-scan sensitivity over basic triple quadrupoles in the same class.
Learn more
Panels of vitamin analysis
Workflow
Comprehensive analysis of both water‑ and fat‑soluble vitamins in human serum requires high sensitivity, robust chromatographic separation, and excellent quantitative performance to address low concentrations, structural similarity, and complex biological matrices. LC‑MS/MS enables confident quantitation of multiple vitamins and metabolites within a single workflow while maintaining reproducibility and throughput for clinical research applications.
Sensitive quantitation of water-soluble vitamins in human serum
Key benefits:
- Rapid protein precipitation sample preparation method
- Sub-ng/mL sensitivity with excellent precision
- Good chromatographic separation of the vitamin panel using the Phenomenex Luna Omega Polar C18 column with an 8 minute runtime
QTRAP 6500+ system
The QTRAP 6500+ system offers revolutionary sensitivity, speed, and performance for your most challenging methods.
Learn more
Sensitive quantitation of fat-soluble vitamins in human serum
Key benefits:
- Fast liquid-liquid extraction sample preparation
- Low-ng/mL sensitivity with excellent precision in serum matrix-calibration standards
- Good chromatographic separation using the Phenomenex Phenyl-hexyl column with a 5 minute runtime
QTRAP 6500+ system
The QTRAP 6500+ system offers revolutionary sensitivity, speed, and performance for your most challenging methods.
Learn more
Featured resources
-
Technical note
Sensitive quantitation of water-soluble vitamins in human serum
In this technical note, the combination of a rapid sample preparation procedure consisting of a protein precipitation with a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled accurate quantitation of 8 water-soluble vitamins extracted from human serum. Excellent linearity was observed across clinically relevant concentrations. Low-ng/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 207:1 for thiamine, 87:1 for riboflavin, 69:1 for nicotinamide, 28:1 for nicotinic acid, 118:1 for pantothenic acid, 9:1 for biotin, 11:1 for cyanocobalamin, and 16:1 for folic acid, demonstrating the quantitative performance of the assay.
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Technical note
Sensitive quantitation of fat-soluble vitamins in human serum
In this technical note, a rapid protein precipitation sample preparation procedure and a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled the accurate quantitation of 6 fat-soluble vitamins in human serum. Excellent linearity was observed across clinically relevant concentrations. Low-nmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 15:1, 34:1, 55:1, 260:1, 1040:1, and 41:1 for retinol, β-carotene, cholecalciferol, ergocalciferol, α-tocopherol, and phylloquinone, respectively. In addition, the method showed excellent precision (<3.1%CV) at low-level concentrations, demonstrating the quantitative performance of the assay.
Comparison of LC-MS/MS solutions
Vitamins | Triple Quad 4500 system | QTRAP 4500 system | SCIEX 5500+ system | SCIEX 5500+ system — QTRAP | Triple Quad 6500+ system | QTRAP 6500+ system | SCIEX 7500+ system | SCIEX 7500+ system — QTRAP |
25-OH Vitamin D3, 25-OH Vitamin D2, 3-epi-25-OH Vitamin D3 | x | x | x | x | x | x | x | x |
Vitamin B1, B6 | x | x | x | x | x | x | x | x |
Vitamin A, E | x | x | x | x | x | x | x | x |
Panel of fat soluble vitamins (Vit D2, D3, A, E, K) |
|
| x | x | x | x | x | x |
Panel of water soluble vitamins (B vitamins) |
|
| x | x | x | x | x | x |
1,25-Dihydroxyvitamin D3, 1,25-Dihydroxyvitamin D2 |
|
|
|
| x | x | x | x |
All resources
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Technical note
Sensitive quantitation of vitamin A and E in human serum
This technical note demonstrates the sensitive and accurate quantitation of vitamins A and E in human serum using a rapid protein precipitation sample preparation procedure with analysis using the SCIEX QTRAP 4500 system. Excellent linearity was observed across clinically relevant concentrations. Low-µmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 72:1 for vitamin A at 0.76 µmol/L and 105:1 for vitamin E at 6.76 µmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, highlighting the assay's strong quantitative performance.
-
Technical note
Quantitative analysis of vitamin A and E in human serum
This technical note demonstrates the sensitive and accurate quantitation of vitamins A and E in human serum using a rapid protein precipitation sample preparation procedure with analysis using the SCIEX QTRAP 4500 system. Excellent linearity was observed across clinically relevant concentrations. Low-µmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 72:1 for vitamin A at 0.76 µmol/L and 105:1 for vitamin E at 6.76 µmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, highlighting the assay's strong quantitative performance.
-
Technical note
Sensitive quantitation of vitamin B1 and B6 in human whole blood
In this technical note, the combination of a rapid sample preparation procedure consisting of a protein precipitation with a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled accurate quantitation of vitamin B1 and B6 extracted from human whole blood. Excellent linearity was observed across clinically relevant concentrations. Low-nmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 769:1 for vitamin B1 at 39 nmol/L and 499:1 for vitamin B6 at 21 nmol/L. In addition, the method showed excellent precision and accuracy at low-level concentrations, demonstrating the quantitative performance of the assay.
-
Technical note
Quantitative analysis of vitamin B1 and B6 in human whole blood
This technical note describes a rapid protein precipitation sample preparation procedure and a robust LC-MS/MS method for the quantitation of vitamins B1 and B6 from human whole blood. Low-nmol/L level sensitivity was shown at the lowest calibrator with signal-to-noise ratios (S/N) of 260:1 for vitamin B1 at 39.0 nmol/L and 45:1 for vitamin B6 at 23.0 nmol/L. The method demonstrated excellent linearity across clinically relevant concentrations as well as excellent precision and accuracy at low-level concentrations, highlighting the assay's quantitative performance.
-
Technical note
Sensitive quantitation of 1,25-dihydroxyvitamin D2 and D3 in human serum
In this technical note, a rapid immunopurification sample preparation procedure and a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ system enabled accurate quantitation of 1,25-dihydroxyvitamin D2 and D3 in human serum. Excellent linearity was observed from 5 to 200 pg/mL. Low-pg/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 20:1 for both 1,25-dihydroxyvitamin D2 and D3 at 5 pg/mL. In addition, the method showed excellent precision (4.8% for 1,25-dihydroxyvitamin D2 and 1.2% for 1,25-dihydroxyvitamin D3) at the lowest calibrator level (5 pg/mL), highlighting the assay's strong quantitative performance.
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Technical note
Quantitative analysis of 25-OH-vitamin D3, 3-epi-25-OH-vitamin D3 and 25-OH-vitamin D2 in human serum
This technical note demonstrates the sensitive and accurate quantitation of 25-OH-vitamin D3, 3-epi-25-OH-vitamin D3 and 25-OH-vitamin D2 in human serum using a liquid-liquid extraction procedure with analysis using the SCIEX QTRAP 4500 system. Excellent linearity was observed across clinically relevant concentrations with r2 values above 0.99 across the calibration range (1-100 ng/mL). The method showed excellent accuracy (93.5% for 25-OH-vitamin D3, 82.7% for 3-epi-25-OH-vitamin D3, and 119.3% for 25-OH-vitamin D2) at the lowest calibrator level (1 ng/mL), highlighting the assay's strong quantitative performance.
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Technical note
Sensitive quantitation of water-soluble vitamins in human serum
In this technical note, the combination of a rapid sample preparation procedure consisting of a protein precipitation with a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled accurate quantitation of 8 water-soluble vitamins extracted from human serum. Excellent linearity was observed across clinically relevant concentrations. Low-ng/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 207:1 for thiamine, 87:1 for riboflavin, 69:1 for nicotinamide, 28:1 for nicotinic acid, 118:1 for pantothenic acid, 9:1 for biotin, 11:1 for cyanocobalamin, and 16:1 for folic acid, demonstrating the quantitative performance of the assay.
-
Technical note
Sensitive quantitation of fat-soluble vitamins in human serum
In this technical note, a rapid protein precipitation sample preparation procedure and a robust and sensitive LC-MS/MS method using the SCIEX QTRAP 6500+ enabled the accurate quantitation of 6 fat-soluble vitamins in human serum. Excellent linearity was observed across clinically relevant concentrations. Low-nmol/L level sensitivity was achieved at the lowest calibrator with signal-to-noise ratios (S/N) of 15:1, 34:1, 55:1, 260:1, 1040:1, and 41:1 for retinol, β-carotene, cholecalciferol, ergocalciferol, α-tocopherol, and phylloquinone, respectively. In addition, the method showed excellent precision (<3.1%CV) at low-level concentrations, demonstrating the quantitative performance of the assay.
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Technical note
Comprehensive analysis of water-soluble and fat-soluble vitamins
Whether you need to quantify water-soluble or fat-soluble vitamins, our cutting-edge analytical solutions offer the precision and sensitivity required to ensure reliable data. Streamline your vitamin analysis workflows and support your clinical research objectives.
Associated applications
Steroid hormones analysis
Analysis of steroid hormones in biological matrices requires high sensitivity and specificity. SCIEX offers cutting-edge LC-MS/MS solutions that deliver the accuracy needed to quantify various therapeutic drugs and their metabolites, ensuring clinicians receive reliable and precise results.
Learn more