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Glycan analysis is crucial to overall biotherapeutic safety. Take for example research scientists performing clone selection. Analytical development researchers running glycan characterization and QA/QC analysts responsible for lost release.
In all these scenarios, determining the exact glycans on large biotherapeutics can be difficult due to their diversity. Different glycan analysis strategies are being applied with varying degrees of success, but few can provide the depth of characterization required with a simple, fast and accurate assay. The downside is that many labs are struggling to cope with the increasing workload.
Accelerate Biotherapeutic Glycan Analysis
With SCIEX fast glycan CE solutions, you can now determine what type and how many glycans are on your large biotherapeutics, up to 2x faster than traditional HILIC methods, with higher resolution. SCIEX LC-MS and CESI-MS solutions can accurately determine intact and subunit level glycoprotein profiles for comparability reasons, as well as obtain site specific glycan information at the peptide level.
Now you can experience the certainty and simplicity of protein glycosylation analysis with innovative LC-MS, CESI-MS, and CE solutions from SCIEX. It has never been easier to gain a comprehensive analysis of glycans on large biotherapeutics in a single solution – with fast, precise and in-depth characterization:
Whether you are using HILIC or CE to quantitatively determine what glycans are present, performing LC-MS or CESI-MS on intact biotherapeutics to determine the relative presence of glycoforms, or analyzing glycopeptides to identify glycans and their location, we have the right solution for you. Our fast glycan technology as well as our best-in-class mass spec options with BioPharmaView software, will simplify analyses and get you quantitative answers faster.
For research use only. Not for use in diagnostic procedures.
The award winning Fast Glycan Technology is up to 2x faster than HILIC. Gain high-resolution N-glycan information for your biotherapeutic protein without the need for long column equilibration, or lengthy sample preparation procedures.
If you need to identify the glycans present on your biotherapeutic protein quantitatively, SCIEX CE technology will enable you to perform released N-glycan analysis in record time.
Includes sample prep reagents and capillary cartridge, with protocols and methods for implementation.
Fast Glycan Software provides high-resolution electropherogram and GU value information along with glycan structural data for identification.
Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.
Delivers rapid glycan heterogeneity identification, capable of profiling your glycans in record time. Combining simplified sample preparation, rapid separations, and automated glycan identification, you can make confident decisions quickly by running glycans up to 2x faster than HILIC with high resolution.
Identify and monitor intact biotherapeutics glycoforms simply, efficiently and accurately with the X500B QTOF LC-MS system. Capture all detectable peptides using SWATH® Acquisition, including the low-level glycopeptides that may have been missed by IDA approaches. Analyse and process large data sets easily using BioPharmaView™ software.
Our award-winning Fast Glycan Technology is up to 2x faster than a HILIC assay. You can gain high-resolution information without the need for long column equilibration, or lengthy sample preparation procedures. The SCIEX solution includes a sample prep kit that is optimized for a 1-hour prep time and is compatible with automation to ensure you can have the fastest possible sample turnaround.
Our Fast Glycan Technology provides immediate N-glycan identification without the need for enzymatic digestion, by using our customizable and flexible database that immediately and accurately identify species based on GU value. This technology will help you to fast-track your analyses and keep up with workloads.
Our QTOF technology provides accurate intact protein data for even very large biotherapeutics. The SCIEX X500B QTOF system with BioPharmaView software has an intuitive user interface, making it simple to set up, run, process and report intact protein glycoforms.
LC-MS of glycopeptides from biotherapeutics is challenging. These species can be present at very low levels and often get missed by traditional IDA based data acquisition approaches. SCIEX QTOF systems use proprietary data-independent SWATH Acquisition that captures data for all detectable peptide species in a sample. Powerful processing with BioPharmaView software confirms the presence or absence of glycopeptides routinely.
If you need to perform routine biotherapeutic glycoform profiling or determine site-specific glycan heterogeneity, the SCIEX X500B QTOF System with BioPharmaView software provides a simple and streamlined solution.
Delivers high accuracy, reliability and repeatability across thousands of injections, with maximum uptime.
Accurate mass analysis with the compact and streamlined QTOF System and the renowned Turbo V™ ionization source.
Intuitive point-and-click acquisition software makes it easier than ever to set-up and run your methods.
Unbiased, data-independent acquisition strategy for analysis of all detectable glycopeptides in a biotherapeutic sample, even those that may be missed by traditional data dependent acquisition methods.
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