Overview
Glycan analysis provides important information on critical quality attributes (CQAs) that could affect stability, safety and efficacy of a protein therapeutic.
Specific needs in understanding the glycosylation profiles change throughout the drug development process, but the requirement for high-resolution glycan information remains the same and is essential to help ensure product quality.
Address pipeline-specific challenges with glycan analysis workflows based on capillary electrophoresis (CE) and mass spectrometry (MS) to support rapid screening, in-depth characterization and lot-to-lot comparisons.
Workflow
Manage development timelines with rapid N-linked glycan analysis
Find a simpler path to market with scalable N-linked glycan analysis solutions that rapidly identify therapeutic antibody glycosylation profiles.
Thousands of samples, including those containing N-linked glycans, must be screened monthly in the early stages of development, driving the need for high-throughput assays to shorten development time.
Meet the need for large-scale sample analysis and glycan profiling throughout development with robust, high-throughput, CE-based workflows that integrate sample preparation, multi-channel separation and screening assays with intuitive data analysis software for rapid reporting.
Manage development timelines with rapid N-linked glycan analysis
Solutions
Workflow
Confirm glycoform identity with detailed glycosylation analysis
Tackle the complex analytical challenges of modern therapeutics.
Intact protein analysis, routinely performed at different stages of monoclonal antibody (mAb) development, is essential for confirming the identity of glycoforms and monitoring product quality. However, increasingly complex glycoform distributions make it challenging to obtain detailed information about glycosylation on complex therapeutic proteins.
LC-MS workflows and data analysis software optimized for biopharma, enable rapid profiling of intact proteins for in-depth characterization. Confident peak assignment and better understanding of the location of glycosylation provide the comprehensive data needed for making critical decisions
Confirm glycoform identity with detailed glycosylation analysis
Solutions
- In-depth characterization of glycosylation profiles
- Post-translational modification (PTM) identification
Workflow
Monitor product quality during manufacturing
Keep pace, with batch comparison workflows designed for confidence in quality.
Routine characterization of biosimilars or batch comparison of manufactured biologic samples is critical for maintaining biotherapeutic quality throughout development and production.
Monitor glycosylation profiles the intact or subunit level with flexible LC-MS solutions providing in-depth characterization and reliable monitoring of known PTMs.
Monitor product quality during manufacturing
Solutions
- Routine glycosylation assays
- High-throughput glycoform screening
All resources
Get insight into high-throughput strategies for glycan analysis to condense development timelines.
See electron activated dissociation (EAD) fragmentation in action for large molecules, and understand the benefit of EAD for identifying and localizing glycosylation.
Learn how electron activated dissociation (EAD) enables near-complete fragmentation of advanced glycation end products (AGEs) while retaining the AGE moieties for confident sequence identification, accurate localization
Enable separation of a 96-well plate in under 2 hours with an N-linked glycan analysis workflow.
Streamline N-linked glycan analysis using automated sample preparation with a magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).
Enable accurate and comprehensive glycopeptide characterization for routine, in-depth analysis of next-generation protein therapeutics and standard monoclonal antibodies (mAbs).
Differentiate positional isomers of O-glycopeptides and leverage electron activated dissociation (EAD) to elucidate complex glycosylation profiles in protein therapeutics.
Associated applications
Subunit mass analysis and middle-down
Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.
Intact protein analysis
Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.
Charge heterogeneity analysis
Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.