Peptide mapping analysis

Achieve new heights in PTM analysis with confidence and speed.

Define critical quality attributes (CQAs) and streamline processes from early to late-stage development with in-depth peptide mapping solutions for next-generation protein therapeutics and standard monoclonal antibodies (mAbs).

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Overview

Develop peptide mapping strategies for the unknown and the routine.

The requirements of peptide mapping analyses change during the drug development process. In early development, full characterization and determining the appropriate product quality attributes (PQAs) are key and depend on extremely complex data processing and analysis. In late development, throughput is a priority to quickly process samples and analyze PQAs. Over the course of biotherapeutic production, continuous monitoring of PTMs and chemical variations is critical to maintain overall product safety and efficacy.

Address the need for complete sequence coverage, comprehensive determination of PTMs and reliable high-throughput monitoring assays with LC-MS solutions designed to address the challenges of peptide mapping at all stages of the therapeutic development pipeline.

Workflow

In-depth peptide mapping and PTM identification

Reach new depths of peptide mapping analysis.
Leverage the power of EAD  to identify and fully characterize potential critical quality attributes (CQAs) and challenging PTMs, such as glycosylation and disulfide linkages, with speed and reproducibility.

Enable in-depth analysis of next-generation protein therapeutics and mAbs with an EAD-based LC-MS platform method for peptide mapping. Visually intuitive pre-built software workflows allow for simple data interpretation and PTM monitoring.

  • High sequence coverage and PTM identification
  • Clear isomer differentiation
  • Localization of glycosylation
  • Disulfide linkage confirmation
  • Simple data interpretation and PTM monitoring

In-depth peptide mapping and PTM identification

Solutions

Suited for:
  • Full understanding of potential CQAs
  • Monitoring of challenging PTMs
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Workflow

Standardize platform peptide mapping and PTM monitoring

Gain confidence in the routine.  Simplify peptide mapping and PTM monitoring to maximize productivity.

Take advantage of pre-built LC-MS workflows and visually intuitive compliance-ready data analysis solutions to speed up routine peptide mapping  and anslysis.

  • Visually intuitive data analysis to monitor standard PTMs
  • Recognize deamidation instantly
  • Identify glycopeptides with confidence
  • Automatically map disulfide bonds
  • Compliance ready

Standardize platform peptide mapping and PTM monitoring

Solutions

Suited for:
  • Routine peptide mapping
  • Monitoring of standard PTMs
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All resources

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Accurate localization of PTMs

Gain the additional power of EAD capabilities for isomer differentiation while maintaining advantages of traditional CID technique.

Comprehensive characterization of protein therapeutics

Dive deeper in to characterization workflows and discuss the advantages of alternative fragmentation techniques EAD to develop a comprehensive understanding of protein therapeutics.

Visualize PTMs throughout development with intuitive data analysis workflows

Distinct workflow structures support diverse analytical biotherapeutic applications, from discovery and development through to production and quality attribute monitoring

Enable differentiation of isomeric amino acids

Superior characterization and monitoring of product quality attributes using an EAD-based multi-attribute method (MAM) to achieve more detail on isomeric amino acids than CID alone.

Achieve accurate localization of PTMs with a single injection platform method.

Leverage the additional power of EAD capabilities for isomer differentiation while maintaining the advantages of traditional CID techniques for comprehensive peptide mapping in less time.

Achieve superior sequence and disulfide linkage confirmation

Maintain labile PTMs for site-specific characterization and perform comprehnsive mapping of etanercept using and EAD-based workflow.

Be agile with flexible workflows to address data analysis needs throughout development.

Distinct workflow structures support diverse analytical biotherapeutic applications from discovery and development to production and quality attribute monitoring.

Routine characterization of biosimilars

Maintain quality for biotherapeutics throughout development and production with a fast, reproducible and robust method for biologic subunit analysis.

Routine peptide mapping with a small footprint

Discover simple solutions for complex characterization challenges.

Associated applications

Multi-attribute methodology

Pursue simpler, faster and more accurate product quality profiles. Reclaim time for innovation and reduce overall analytical testing burden with software solutions for product quality attribute (PQA) monitoring.

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Subunit mass analysis and middle-down

Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.

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Intact protein analysis

Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.

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