Glycan analysis

Fully understand drug candidate glycoprofiles with pipeline-specific solutions. 

Gather critical high-resolution information and site-specific data points with advanced glycan analysis for complex biologic development.

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Overview

Glycan analysis provides important information on critical quality attributes (CQAs) that could affect stability, safety and efficacy of a protein therapeutic.

Specific needs in understanding the glycosylation profiles change throughout the drug development process, but the requirement for high-resolution glycan information remains the same and is essential to help ensure product quality.

Address pipeline-specific challenges with glycan analysis workflows based on capillary electrophoresis (CE) and mass spectrometry (MS) to support rapid screening, in-depth characterization and lot-to-lot comparisons.

Workflow

Manage development timelines with rapid N-linked glycan analysis

Find a simpler path to market with scalable N-linked glycan analysis solutions that rapidly identify therapeutic antibody glycosylation profiles.

Thousands of samples, including those containing N-linked glycans, must be screened monthly in the early stages of development, driving the need for high-throughput assays to shorten development time. 

Meet the need for large-scale sample analysis and glycan profiling throughout development with robust, high-throughput, CE-based workflows that integrate sample preparation, multi-channel separation and screening assays with intuitive data analysis software for rapid reporting.

  • Streamlined sample preparation
  • Rapid method development with parallel processing
  • High-throughput, parallel screening of released N-linked glycans
  • Reproducible migration times and accurate glycan peak assignments
  • Ready for integration into automation
Workflow Section 1 - 1

Manage development timelines with rapid N-linked glycan analysis

Solutions

Suited for:
  • High-throughput glycan screening
  • Rapid clone selection
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Workflow

Confirm glycoform identity with detailed glycosylation analysis

Tackle the complex analytical challenges of modern therapeutics.

Intact protein analysis, routinely performed at different stages of monoclonal antibody (mAb) development, is essential for confirming the identity of glycoforms and monitoring product quality. However, increasingly complex glycoform distributions make it challenging to obtain detailed information about glycosylation on complex therapeutic proteins.

LC-MS workflows and data analysis software optimized for biopharma, enable rapid profiling of intact proteins for in-depth characterization. Confident peak assignment and better understanding of the location of glycosylation provide the comprehensive data needed for making critical decisions

  • Accurate localization of glycosylation
  • Confident differentiation of position isomers
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Workflow Section 2 - 2

Confirm glycoform identity with detailed glycosylation analysis

Solutions

Suited for:
  • In-depth characterization of glycosylation profiles
  • Post-translational modification (PTM) identification
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Workflow

Monitor product quality during manufacturing

Keep pace, with batch comparison workflows designed for confidence in quality.

Routine characterization of biosimilars or batch comparison of manufactured biologic samples is critical for maintaining biotherapeutic quality throughout development and production.

Monitor glycosylation profiles the intact or subunit level with flexible LC-MS solutions providing in-depth characterization and reliable monitoring of known PTMs.

  • Simple and accurate monitoring and identification of intact glycoforms
  • Capture of all common detectable peptides, including low-level glycopeptides
  • Ability to analyze and process large data sets
Workflow Section 3 - 1
Workflow Section 3 - 2

Monitor product quality during manufacturing

Solutions

Suited for:
  • Routine glycosylation assays
  • High-throughput glycoform screening
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All resources

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Facilitate clone selection with high-throughput glycan profiling

Get insight into high-throughput strategies for glycan analysis to condense development timelines.

EAD fragmentation explained

See electron activated dissociation (EAD) fragmentation in action for large molecules, and understand the benefit of EAD for identifying and localizing glycosylation.

Confident identification and characterization of advanced glycation end products

Learn how electron activated dissociation (EAD) enables near-complete fragmentation of advanced glycation end products (AGEs) while retaining the AGE moieties for confident sequence identification, accurate localization

Reproducible glycan analysis for clone selection

Enable separation of a 96-well plate in under 2 hours with an N-linked glycan analysis workflow.

Eliminate labor-intensive bench work and provide a higher throughput in N-linked glycan analysis with highly consistent results.

Streamline N-linked glycan analysis using automated sample preparation with a magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).

Accurate peak assignment becomes less daunting with detailed information about glycosylation on complex therapeutic proteins

Enable accurate and comprehensive glycopeptide characterization for routine, in-depth analysis of next-generation protein therapeutics and standard monoclonal antibodies (mAbs).

Facilitate in-depth characterization of therapeutic proteins

Differentiate positional isomers of O-glycopeptides and leverage electron activated dissociation (EAD) to elucidate complex glycosylation profiles in protein therapeutics.

Advanced tools for identifying and monitoring CQAs

Reliable processing, visualization, reviewing and reporting tools offer an easy path for decision making in biopharmaceutical development.

Associated applications

Subunit mass analysis and middle-down

Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.

Learn more
Intact protein analysis

Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.

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Charge heterogeneity analysis

Pursue the end goal with confidence. Achieve the full picture and accelerate decisions with comprehensive characterization of biopharmaceutical charge variants.

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