Biopharma

Monday, June 5

235 Enhanced electron-anion interaction in electron-cation neutral plasma: Sequencing of oligonucleotides by electron-detachment dissociation

Takashi Baba¹; Kaoru Karasawa²; Eva Duchoslav¹

¹SCIEX; ² SCIEX Japan

 

393 A complete solution for sequence variant analysis of recombinant antibodies and therapeutic proteins with electron-activated dissociation and automatic data processing

Zhengwei Chen; Lei Xiong

SCIEX

 

603 Confident sequence analysis of a trispecific antibody using an electron-activated dissociation (EAD)-based middle-down workflow

Andrew Mahan¹; Hirsh Nanda¹; Haichuan Liu²; Zoe Zhang²

¹Johnson & Johnson Pharmaceutical Research & Development; ²SCIEX

 

627 A disruptive approach to the characterization of mAb charge variants during process development

Jason Barker¹; Scott Mack²; Maggie A. Ostrowski²;  Greg Adams¹

¹Fujifilm Diosynth Biotechnologies USA,; ²SCIEX

 

628 Top/middle-down protein sequencing: A novel automated data processing tool for the top-down/middle-down analysis of biological therapeutics

Mona Hamada¹; Stefano Gotta²; Wen Jin¹; Amy Claydon³; Amandine Boudreau¹

¹SCIEX; ²Genedata, Switzerland; ³Genedata, United Kingdom

 

670 Identification and differentiation of positional isomers of O-linked glycopeptides of etanercept using an alternative electron-based MS/MS approach

Haichuan Liu¹; Xuezhi Bi²; Elliott Jones¹; Zoe Zhang¹

¹SCIEX; ²Bioprocessing Technology Institute (BTI), Singapore

 

671 A single-injection workflow for enhanced peptide mapping using collision-induced dissociation (CID) and electron activated dissociation (EAD)

Haichuan Liu; Elliott Jones; Zoe Zhang

SCIEX

 

704 Systematic optimization of electron activated dissociation for top-down targeted protein sequencing

Jason Causon; Ihor Batruch

SCIEX

 

Tuesday, June 6

008 Comprehensive characterization of an engineered Cas9 protein and its post-translational modifications (PTMs) by LC-MS/MS

Zhichang Yang¹; Sahana Mollah¹; Chao-Xuan Zhang²; Alicia Powers²; Yan Lu²

¹SCIEX; ²St. Jude Children's Research Hospital

 

205 Characterization of proteins in influenza virus vaccines by LC-MS/MS and CGE

Xiaoxia Zhang¹; Yang Song¹; Ji Luo¹; Hongxu Chen¹; Zoe Zhang²

¹SCIEX China; ²SCIEX

 

289 In-depth O-glycosylation characterization and comparison of commercially available etanercept products using the ZenoTOF 7600 system

Wentao Wang¹; Hexing Song¹; Ji Luo¹; Hongxu Chen¹; Lihai Guo¹; Zoe Zhang²; Xiang Li³

¹SCIEX China; ²SCIEX; ³NIFDC China

 

414 Using a branched RF-ion trap to combine EAD, ultraviolet-photodissociation (UVPD) and CID fragmentation

Mircea Guna; Pavel Ryumin; Anjali Chelur; Nick Albeanu; Takashi Baba; J.C. Yves Le Blanc

SCIEX

 

514 Molecular weight confirmation and impurity analysis of a 70-mer oligonucleotide

Sahana Mollah¹; Kerstin Pohl¹; Remco van Soest¹; Ling Huang²

¹SCIEX; ²Integrated DNA Technologies

 

605 Development of a streamlined single-injection workflow for middle-down analysis of protein therapeutics using electron activated dissociation (EAD)

Haichuan Liu; Elliott Jones; Zoe Zhang

SCIEX

 

662 Protein cooking: Combining thermal-CID and EAD for intact protein sequencing

Cuong Le; Patrick Pribil; J.C. Yves Le Blanc

SCIEX

 

665 Top-down analysis of intact NISTmAb and CA (II) by capillary isoelectric focusing coupled to electron capture dissociation mass spectrometry

Stanislav S. Beloborodov; Pavel Ryumin; Wen Jin; Bradley B. Schneider; Bill Loyd

SCIEX

 

666 Leveraging intrachain disulfide bonds for improved sequence coverage in electron capture dissociation (ECD) middle-down analysis

Wen Jin; Pavel Ryumin; Lyle L. Burton; Takashi Baba

SCIEX

 

Wednesday, June 7

030 Charge heterogeneity characterization and peak identification of complex protein therapeutics using icIEF-UV/MS

Mingjie Cui¹; Kristin Schultz-Kuszak¹; Trust Razunguzwa¹; Weiguo Zhai¹; Scott Mack²; Jingwen Ding²; Zoe Zhang²; Maggie Ostrowski²

¹AstraZeneca; ²SCIEX

 

032 In-depth characterization of protein-based therapeutics using collision-induced dissociation (CID) and electron-activated dissociation (EAD)

Jared Kress¹; Haichuan Liu²; Zoe Zhang²; Hari Kosanam*¹

¹Merck & Co., Inc.; ²SCIEX

 

035 Probing the cause of antibody discoloration via relative quantification of glycation and associated species using an EAD-based multi-attribute methodology (MAM)

Haichuan Liu*¹; Marcia Santos¹; Andrew Mahan²; Hirsh Nanda²; Kristen Nields²; Chelsea Lenonce²; Zoe Zhang¹

¹SCIEX; ²Janssen

 

174 Unraveling sequences of impurities and degradants of non-linear therapeutic peptides using a high-resolution LC-MS/MS workflow with electron activated dissociation

Eva Duchoslav¹; Harini Kaluarachchi¹; Giulia Calloni²

¹SCIEX; ²SCIEX Germany

 

413 Localization of labile tyrosine sulfation using an alternative electron-based MS/MS approach in positive and negative ion modes

Haichuan Liu; Takashi Baba; Elliott Jones; Mark Lies; Zoe Zhang

SCIEX

 

519 Comprehensive characterization of the lipid nanoparticle (LNP) ALC-0315 and its impurities using electron-activated dissociation (EAD)

Zhichang Yang¹; Paul Baker¹; Sahana Mollah¹; Robert Proos¹; Jon Le Huray²

¹SCIEX; ²Acuitas Therapeutics Inc.

 

Thursday, June 8

10:10 a.m. Oral Presentation Characterization of sialylated N-glycopeptide isomers on reversed phase LC-EAD mass spectrometry

Suya Liu; Zoe Zhang; Pavel Ryumin; Takashi Baba

SCIEX

 

557 High resolution and sensitive LC-MS/MS method for the quantitation of oligonucleotides

Dilipkumar Reddy Kandula; Greg Roman; Lei Xiong

SCIEX

 

 

 

Clinical Research

Monday, June 5

117 Determination of 18 steroid hormones in human serum using rapid protein precipitation method coupled with liquid chromatography-tandem mass spectrometry

Dan Liu; Fengmei Hu; Chao Huang; Xianglong Zhao; Lihai Guo

SCIEX China

 

124 Simultaneous quantitation of renin activity, aldosterone and angiotensin II in human plasma using rapid protein precipitation with the Citrine Triple Quad system from SCIEX

Fengmei Hu; Dan Liu; Chao Huang; Xianglong Zhao; Lihai Guo

SCIEX China

 

131 Determination of nine neurotransmitters in plasma by HPLC ESI-MS/MS

Ruichen Liu; Xuzhe Pei; Chao Huang; Xianglong Zhao; Lihai Guo

SCIEX China

 

628 Top/middle-down protein sequencing: A novel automated data processing tool for the top-down/middle-down analysis of biological therapeutics

Mona Hamada¹; Stefano Gotta²; Wen Jin¹; Amy Claydon³; Amandine Boudreau¹

¹SCIEX; ²Genedata, Switzerland; ³Genedata, United Kingdom

 

 

 

 

Environmental and industrial

Tuesday, June 6

230 An ultra-high sensitivity analysis of PFAS compounds in multiple water sources using the SCIEX 7500 system

Bertram Nieland¹; Jack Steed²; Rens-Jan Jaspers³; Kay Hup³; Abdessamad Chahbouni³; Jianru Stahl-Zeng⁴

¹SCIEX The Netherlands; ²SCIEX United Kingdom; ³Het Waterlaboratorium, The Netherlands; ⁴SCIEX Germany

 

 

 

 

Food and beverage

Wednesday, June 7

590 Tracing the origin of Chinese lotus root varieties using high-resolution mass spectrometry

Jiao Liu¹; Jinmei Chen²; Liuqing Zhao²; Zong Yang²; Bingjie Liu²; Lihai Guo²

¹Hubei Academy of Agricultural Sciences, China; ²SCIEX China

 

Thursday, June 8

127 The application of a QTOF instrument to enable traceability of the origin of ginger

Xiaogang Zhang; Liuqing Zhao; Zong Yang; Bingjie Liu; Lihai Guo

SCIEX China

 

132 Tracing the origins of red cabbage moss using a QTOF instrument for rapid nutrient identification

Qing Liu¹; Liuqing Zhao¹; Ming Yang²; Zong Yang¹; Bingjie Liu¹; Lihai Guo¹

¹SCIEX China; ²Wuhan Food and Cosmetics Inspection Institute, China

 

503 Application of a non-targeted metabolomics approach to identify the aging time of yellow rice wine

Huimin Chen¹; Jiangang Hu²; Zong Yang¹; Bingjie Liu¹; Lihai Guo¹

¹SCIEX China; ²Shaoxing Food and Drug Administration, China

 

 

 

Life science research

Monday, June 5

576 High-throughput phosphoproteomics of formalin-fixed, paraffin-embedded rat tissues using microflow Zeno SWATH

Erin M. Humphries^{1, 2}; Dylan Xavier¹; Keith Ashman³; Peter G. Hains¹; Phillip J. Robinson^{1, 2}

¹Children's Medical Research Institute, Australia; ²The Faculty of Medicine and Health, The University of Sydney, Australia; ³SCIEX Australia

 

704 Systematic optimization of electron activated dissociation for top-down targeted protein sequencing

Jason Causon; Ihor Batruch

SCIEX

 

Tuesday, June 6

122 Molecular landscapes of breast cancer subtypes: Data independent acquisition and identification of potential targets for stromal reprogramming

Jordan B. Burton¹; Deng Pan²; Joanna Bons¹; Rosemary Bai²; Chira Chen-Tanyolac²; Christie L. Hunter³; Philippe Gascard²; Thea D. Tlsty²; Birgit Schilling¹

¹Buck Institute for Research on Aging; ²University of California San Francisco; ³SCIEX

 

414 Using a branched RF-ion trap to combine EAD, ultraviolet-photodissociation (UVPD) and CID fragmentation

Mircea Guna; Pavel Ryumin; Anjali Chelur; Nick Albeanu; Takashi Baba; J.C. Yves Le Blanc

SCIEX

 

466 Analysis of short-chain fatty acids (SCFAs) by LC-MS/MS coupled with chemical derivatization

Hanpeng Jiang; Zhimin Long; Lihai Guo

SCIEX China

 

467 Rapid analysis of 11 energy-rich phosphate compounds using the SCIEX Triple Quad 6500+ system

Junmiao Chen; Dandan Si; Zhimin Long

SCIEX China

 

469 Targeted analysis of energy metabolites in cells by using SCIEX LC-MS/MS

Xiaoyuan Shi; Zhimin Long; Lihai Guo

SCIEX China

 

514 Molecular weight confirmation and impurity analysis of a 70-mer oligonucleotide

Sahana Mollah¹; Kerstin Pohl¹; Remco van Soest¹; Ling Huang²

¹SCIEX; ²Integrated DNA Technologies

 

662 Protein cooking: Combining thermal-CID and EAD for intact protein sequencing

Cuong Le; Patrick Pribil; J.C. Yves Le Blanc

SCIEX

 

680 Enabling high throughput proteomics with short gradients and Zeno SWATH DIA by utilising the speed and sensitivity of the ZenoTOF 7600 system

Dylan Xavier¹; Keith Ashman²; Peter G. Hains¹; Phillip J. Robinson¹

¹ProCan®, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Australia; ²SCIEX Australia

 

Wednesday, June 7

537 A comparative study on the identification of plasma lipids using collision-induced dissociation and electron-activated dissociation

Kiran Maan¹; Dipankar Malakar¹; Cagakan Ozbalci²

¹SCIEX India; ²SCIEX United Kingdom

 

544 High-coverage targeted lipidomics analysis by LC-MS/MS in mouse brain tissue prepared by laser-capture microdissection

Chenchun Zhong¹; Zhimin Long¹; Tianlong Gao²; Lihai Guo¹

¹SCIEX China; ²Leica Microsystems China

 

545 Identification and quantification of unsaturated fatty acids using electron-activated dissociation (EAD) fragmentation on the ZenoTOF 7600 system

Zhuo Man; Dandan Si; Zhimin Long

SCIEX China

 

624 Label-free quantitation of protein mixtures using Zeno SWATH data-independent acquisition (DIA)

Randy J Arnold¹; Takeshi Shibata²; Ushio Takeda²; Haruka Kumabe³; Takeshi Masuda⁴; Sumio Ohtsuki³; Patrick Pribil¹

¹SCIEX; ²SCIEX Japan; ³Kumamoto University, Japan; ⁴Keio University, Japan

 

Thursday, June 8

10:10 a.m. Oral Presentation Characterization of sialylated N-glycopeptide isomers on reversed phase LC-EAD mass spectrometry

Suya Liu; Zoe Zhang; Pavel Ryumin; Takashi Baba

SCIEX

 

027 Quantifying 1000 protein groups per minute of LC gradient using Zeno SWATH data-independent acquisition (DIA)

Nick Morrice¹; Ihor Batruch²; Patrick Pribil²

¹SCIEX United Kingdom; ²SCIEX

 

028 Integrating DIA-NN software analysis of SWATH data-independent acquisition (DIA) data into a cloud processing pipeline

Melanie Juba¹; Nick Morrice²; Alexandra Antonoplis¹; Christie Hunter¹

¹SCIEX; ²SCIEX United Kingdom

 

042 Exploring the benefits of differential mobility separation (DMS) and SWATH data independent acquisition (DIA) for complex proteomic sample analysis

J.C. Yves Le Blanc; Eva Duchoslav; Lyle Burton; Stephen Tate; Bradley Schneider

SCIEX

 

456 Establishment of a global metabolomics method for Arabidopsis thaliana using high-sensitivity MS/MS on the ZenoTOF 7600 system

Jinmei Chen; Dandan Si; Zhimin Long; Lihai Guo

SCIEX China

 

677 Ultrahigh-throughput peptide quantitation of an acute phase protein panel using Acoustic Ejection Mass Spectrometry (AEMS) and peptide enrichment

Christie L. Hunter¹; Bart Van Puyvelde^2,3; Oliver Wang³; Maxim Zhgamadze³; Qin Fu³; Esthelle Hoedt³; Maarten Dhaenens²; Dieter Deforce²; Jennifer E. Van Eyk³

¹SCIEX; ²ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Belgium; ³Cedars-Sinai Medical Center

 

 

 

Pharma

Monday, June 5

3:50 p.m. Oral Presentation A powerful single method for sensitive quantitation and targeted/non-targeted identification of cell culture media (CCM) components using accurate mass spectrometry

Marialuce Maldini¹; Antonella Chiapparino²; Eshani Nandita³

¹SCIEX Italy; ²SCIEX Germany; ³SCIEX

 

312 Acoustic Ejection Mass Spectrometry for high-throughput compound QC

Chang Liu¹; Alandra Quinn²; Gordana Ivosev¹; Thomas R. Covey¹; Brendon Kapinos²

¹SCIEX; ²Pfizer

 

628 Top/middle-down protein sequencing: A novel automated data processing tool for the top-down/middle-down analysis of biological therapeutics

Mona Hamada¹; Stefano Gotta²; Wen Jin¹; Amy Claydon³; Amandine Boudreau¹

¹SCIEX; ²Genedata, Switzerland; ³Genedata, United Kingdom

 

Tuesday, June 6

8:50 a.m. Oral Presentation Confident characterization and identification of glucuronide metabolites using diagnostic fragments from orthogonal MS/MS data

Rahul Baghla; Eshani Nandita

SCIEX

 

662 Protein cooking: Combining thermal-CID and EAD for intact protein sequencing

Cuong Le; Patrick Pribil; J.C. Yves Le Blanc

SCIEX

 

Wednesday, June 7

047 Sensitive bioanalysis of galactosyl sphingosine (GalSPH) and glucosyl sphingosine (GluSPH) in cerebral spinal fluid

Kean Woodmansey¹; Jack Steed¹; Jessica Smith¹; Freddy Oostebring²; Jianru Stahl-Zeng³

¹SCIEX United Kingdom; ²Ardena Bioanalysis, Netherlands; ³SCIEX Germany

 

140 Automated workflow to study microsomal clearance and analysis of metabolites using collision-induced dissociation and electron-activated dissociation MS/MS data

Bahar Rizi; Rahul Baghla; Eshani Nandita

SCIEX

 

147 Open port sample injector with electromagnetic mixing for an affinity selection MS system

Thomas Covey¹; Chang Liu¹; Peter Kovarik¹; Purkayastha (Babu) Subhasish¹; Sasi Pillai¹; Gary Van Berkel²; Emmet Welch³; Jianli Zhao³; Sam Michaels⁴; John Janizewski⁴; Meghav Verma⁴; Jonathan Shrimp⁴; Erik Miller⁵

¹SCIEX; ²GVB Ventures; ³Phenomenex; ⁴National Center For Advancing Translational Sciences; ⁵IMI Adaptas

 

148 Well-based method scheduling for high-throughput mass spectrometry

David Cox; Bogdan Georgescu; Adam Latawiec; Chang Liu

SCIEX

 

174 Unraveling sequences of impurities and degradants of non-linear therapeutic peptides using a high-resolution LC-MS/MS workflow with electron activated dissociation

Eva Duchoslav¹; Harini Kaluarachchi¹; Giulia Calloni²

¹SCIEX; ²SCIEX Germany

 

Thursday, June 8

228 Rapid and high-throughput screening of 3 cannabinoids in cell culture medium using the Echo® MS system from SCIEX

Pengyi Hou; Dandan Si; Zhimin Long; Lihai Guo

SCIEX China

 

715 Quantification of boronic acids at pg/mL levels of sensitivity

Junmiao Chen¹; Dandan Si¹; Zhimin Long¹; Jack Steed²

¹SCIEX China; ²SCIEX United Kingdom

 

716 Sensitive quantitation of the proteolysis targeting chimera (PROTAC) TL 13-112 in rat plasma using an LC-MS/MS workflow

Ebru Selen; Rahul Baghla; Eshani Nandita

SCIEX

 

718 A sensitive method for the quantitation of formoterol in human plasma

Sashank Pillai¹; Lakshmanan Deenadayalan¹; Rahul Baghla²; Elliott Jones²; Eshani Nandita²

¹SCIEX India; ²SCIEX

 

719 Low-level quantitation of 10 mutagenic nitrosamine impurities in pioglitazone hydrochloride using accurate mass spectrometry

Eshani Nandita¹; Rahul Baghla¹; Lakshmanan Deenadayalan²; Jack Steed³

¹SCIEX; ²SCIEX India; ³SCIEX United Kingdom

 

 

 

 

Technology

Tuesday, June 6

414 Using a branched RF-ion trap to combine EAD, ultraviolet-photodissociation (UVPD) and CID fragmentation

Mircea Guna; Pavel Ryumin; Anjali Chelur; Nick Albeanu; Takashi Baba; J.C. Yves Le Blanc

SCIEX

 

662 Protein cooking: Combining thermal-CID and EAD for intact protein sequencing

Cuong Le; Patrick Pribil; J.C. Yves Le Blanc

SCIEX

 

Wednesday, June 7

147 Open port sample injector with electromagnetic mixing for an affinity selection MS system

Thomas Covey¹; Chang Liu¹; Peter Kovarik¹; Purkayastha (Babu) Subhasish¹; Sasi Pillai¹; Gary Van Berkel²; Emmet Welch³; Jianli Zhao³; Sam Michaels⁴; John Janizewski⁴; Meghav Verma⁴; Jonathan Shrimp⁴; Erik Miller⁵

¹SCIEX; ²GVB Ventures; ³Phenomenex; ⁴National Center For Advancing Translational Sciences; ⁵IMI Adaptas

 

Thursday, June 8

042 Exploring the benefits of differential mobility separation (DMS) and SWATH data independent acquisition (DIA) for complex proteomic sample analysis

J.C. Yves Le Blanc; Eva Duchoslav; Lyle Burton; Stephen Tate; Bradley Schneider

SCIEX