Peptide mapping analysis

Achieve new heights in PTM analysis with confidence and speed.

Define critical quality attributes (CQAs) and streamline processes from early to late-stage development with in-depth peptide mapping solutions for next-generation protein therapeutics and standard monoclonal antibodies (mAbs).

Overview

Develop peptide mapping strategies for the unknown and the routine.

The requirements of peptide mapping analyses change during the drug development process. In early development, full characterization and determining the appropriate product quality attributes (PQAs) are key and depend on extremely complex data processing and analysis. In late development, throughput is a priority to quickly process samples and analyze PQAs. Over the course of biotherapeutic production, continuous monitoring of PTMs and chemical variations is critical to maintain overall product safety and efficacy.

Address the need for complete sequence coverage, comprehensive determination of PTMs and reliable high-throughput monitoring assays with LC-MS solutions designed to address the challenges of peptide mapping at all stages of the therapeutic development pipeline.

In-depth peptide mapping and PTM identification

Workflow

Reach new depths of peptide mapping analysis.
Leverage the power of EAD  to identify and fully characterize potential critical quality attributes (CQAs) and challenging PTMs, such as glycosylation and disulfide linkages, with speed and reproducibility.

Enable in-depth analysis of next-generation protein therapeutics and mAbs with an EAD-based LC-MS platform method for peptide mapping. Visually intuitive pre-built software workflows allow for simple data interpretation and PTM monitoring.

  • High sequence coverage and PTM identification
  • Clear isomer differentiation
  • Localization of glycosylation
  • Disulfide linkage confirmation
  • Simple data interpretation and PTM monitoring

In-depth peptide mapping and PTM identification

Suited for:

  • Full understanding of potential CQAs
  • Monitoring of challenging PTMs
ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

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ZenoTOF 7600 system

A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology and a step-change in data independent acquisition.

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Biologics Explorer software

Be empowered with highly accurate and informative workflows for fully characterizing protein therapeutics and viral vector proteins.

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Standardize platform peptide mapping and PTM monitoring

Workflow

Gain confidence in the routine.  Simplify peptide mapping and PTM monitoring to maximize productivity.

Take advantage of pre-built LC-MS workflows and visually intuitive compliance-ready data analysis solutions to speed up routine peptide mapping  and anslysis.

  • Visually intuitive data analysis to monitor standard PTMs
  • Recognize deamidation instantly
  • Identify glycopeptides with confidence
  • Automatically map disulfide bonds
  • Compliance ready

Standardize platform peptide mapping and PTM monitoring

Suited for:

  • Routine peptide mapping
  • Monitoring of standard PTMs
ExionLC AE system

Reproducibility, reliability and carryover performance to match your quantitative workflows. Dependability you can count on, from injection to injection and batch after batch.

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ExionLC AE system

A purpose-built QTOF system, designed specifically to accelerate everyday biologics characterization.

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SCIEX OS software

Unleash the analytical power of the next-generation software platform for data acquisition and processing.

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Associated applications

Multi-attribute methodology

Pursue simpler, faster and more accurate product quality profiles. Reclaim time for innovation and reduce overall analytical testing burden with software solutions for product quality attribute (PQA) monitoring.

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Subunit mass analysis and middle-down

Take control of the unknowns. Gain confident sequence confirmation and localization of post-translational modifications (PTMs) at the sub-unit level.

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Intact protein analysis

Confidently select lead candidates and ensure reproducible product quality. Discover fast and reliable solutions that simplify identification and characterization of complex molecules at the intact level.

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