The Power of Precision
cIEF and CZE Charge Heterogeneity Analysis of Biologics
When working with a monoclonal antibody, you need to know the different charge variants to determine identity and stability. You also need the utmost confidence in your results, as the success of your biologic can depend on the accuracy of your results.
Whether you have several methods to develop, samples to QC with limited resources or labs around the world that all need to use the same methods, you need a solution that you can trust. You need to develop charge heterogeneity methods with high resolution and precision that are fast and easy to use, as well as easily transferable across the globe.
The SCIEX PA 800 Plus allows you to confidently assess charge heterogeneity with ultra-high resolution (CIEF) or simplify your sample preparation (CZE). The PA 800 Plus simplifies your workflows and streamlines your methods so you can improve lab productivity and performance. The system also provides you with the raw data required for regulatory acceptance.
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Quick and Easy Charge Heterogeneity Analysis by CZE
Capillary zone electrophoresis (CZE) results in faster separation compared to liquid chromatography, reduced sample preparation complexity, and minimal required optimization – all with high reproducibility.
Maximize Resolution with cIEF
For maximum resolution, capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units.
Which is Right for Your Lab?
Watch the Webinar: Charge Heterogeneity Analysis of Biologics by High Resolution cIEF and High Throughput CZE Methods
Video Length
Labs that need to analyze mAbs for identity and stability can rapidly obtain high resolution charge heterogeneity information with simple sample preparation, using CZE. You can also develop methods with high resolution and precision, that are fast and easy to use, as well as easily transferable across the globe.
PA 800 Plus
Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.
EZ-CE Cartridge
With an inventory of just the EZ-CE cartridge you can run CE-SDS, Fast Glycan, and Rapid Charge Variant analyses.
CZE Rapid Charge Variant Analysis Kit
Charge variant analysis has never been easier. No sample buffer is required; simply dilute the sample in water and you are ready for your separation.
Webinar
Intercompany Study to Evaluate the Robustness of CZE Technology for the Heterogeneity Analysis of mAbs
For maximum sensitivity, ultra-high-resolution capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units. The SCIEX cIEF workflow on the PA 800 Plus System has proven both robust and portable. Universal or platform methods can also be created – significantly decreasing method development efforts and simplifying workflows with a single method for molecules across a wide pI range.
PA 800 Plus
Flexible platform for multiple analytical assays with a temperature controlled capillary for the utmost reproducibility.
Advanced cIEF Starter Kit
With an inventory of just the EZ-CE cartridge you can run CE-SDS, Fast Glycan, and Rapid Charge Variant analyses.
pI Marker Kit
pI Marker Kit features synthetic peptides for high resolution and quantitative reproducibility in calculating protein pI.
Technical Note
High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10
Technical Note
Achieving Robust cIEF Analysis of Monoclonal Antibodies While Increasing Capillary Run-Life and Maintaining High Resolution
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