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Biomarkers
Surfactant protein markers quantified with an antibody-free platform
Sensitive bioanalysis of galactosyl sphingosine (GalSPH) and glucosyl sphingosine (GluSPH) in cerebral spinal fluid
Quantified CSF markers for lysosomal storage disease
Sensitive multiplexed quantitation of protein biomarkers for early drug discovery and development. Low-ng/mL LLOQs were achieved for the 4 surfactant proteins analyzed in human plasma
Biotherapeutics
Immunoaffinity-LC-MRM quantitation of ADCs
Ultra-sensitive LC-MRM analysis for trastuzumab-emtansine quantitation in rat plasma
Quantifying combination mAb therapeutics with multiplexed accurate mass LC-MS/MS
Simultaneous quantitation of trastuzumab and pertuzumab in human serum using accurate mass spectrometry achieved LLOQs of 0.15 μg/mL and strong linearity, with a wide linear dynamic range (LDR) of 3.3 orders of magnitude
High sensitivity LC-MRM workflow for signature peptide quantitation
A new method for increased sensitivity in peptide quantitation is used on a series of surrogate peptides in rat plasma. Ultra-low LLOQs ranging from 5 to 39 fmol/mL are achieved with the SCIEX 7500 system
Cyclic peptides
Low-level quantitation of cyclic peptides using the M5 microLC system
Cyclic peptide quantitation using a microflow trap-and-elute method. Low-pg/mL quantitation was achieved for human atrial natriuretic peptide (ANP) in rat plasma
Improved LC-MRM quantitation sensitivity of cyclic peptides (ANP and NP)
Improved LC-MRM quantitation sensitivity for cyclic peptides from the natriuretic peptide family
Low-pg/mL quantitation of cyclic peptides in rat plasma using microflow LC
The quantitation of cyclic peptides using a microflow trap-and-elute method
HPAPI
Sensitive quantitation of the PROTAC degrader ARV-110 in rat plasma
Sensitive analysis of the proteolysis targeting chimera (PROTAC) degrader ARV-110 in rat plasma
High potency drug candidates with improved sensitivity in in-vitro transporter assays
Use of lower compound concentration in transporter assays results in more accurate data that can guide early stage drug development
Low pg/mL quantitation of TL 13-112, a protein targeting chimera (PROTAC) in rat plasma
Highly sensitive, robust, rapid workflow to quantify TL 13-112 and its inactive control, TL 13-110, in rat plasma
A sensitive method for the quantitation of bimatoprost in human plasma
A sensitive method for the quantitation of bimatoprost in human plasma
Next-level sensitivity for the quantitation of warfarin and furosemide in human plasma
Sensitive quantitation of small molecule pharmaceutical compounds, extracted from human plasma, using minimal sample preparation and negative ion mode-based analysis
Inhaled products
Routinely detect ultra-low levels of two highly potent inhaled compounds
Quantifying fluticasone propionate and salmeterol xinafoate with high sensitivity in human plasma
A sensitive method for the quantitation of mometasone furoate in human plasma
A sensitive method for quantifying a highly potent, orally inhaled drug, mometasone furoate, in human plasma
A sensitive method for the quantitation of formoterol in human plasma
A sensitive method for the quantitation of formoterol, in human plasma, employing a simple liquid-liquid extraction sample preparation
Insulin and Insulin Analogs
Quantitative analysis of IGF-1 and 2 by LC-MRM with scheduled ionization
Quantitation of human insulin-like growth factor-1 and 2 by MRM LC-MS/MS with scheduled ionization
Highly sensitive quantitative workflow for insulin analogs with minimal sample prep
Using intact insulin degludec in rat plasma to demonstrate an LLOQ of 25 pg/mL, with simple protein precipitation sample preparation and an 8-minute LC-MS/MS analysis
Achieve low-pg/mL level quantitation of insulin lispro in rat plasma
Using intact insulin lispro in rat plasma to demonstrate an LLOQ of 50 pg/mL, with simple sample preparation and an 8-minute LC-MS/MS analysis
Oligonucleotides
Ultra-high sensitivity for quantitation of ASO in human plasma
4-fold increase in S/N when quantifying an anti-sense oligonucleotide (ASO) with the new SCIEX 7500 system!
Ultra-low LLOQs for ASOs in rat plasma using a trap-and-elute microflow method
20x improvement in sensitivity for antisense oligonucleotides (ASOs) in rat plasma using a microflow trap-and-elute method
Dos and don'ts of oligonucleotide analysis
Key points to keep in mind when developing your oligonucleotide analysis process
Peptide quant
Superior sensitivity for peptide quantitation in matrix
Quantifying peptides at limits as low as 1 amol, with high accuracy, linearity, and reproducibility
Nitrosamines
The stability of an LC-MS/MS system over several months of use highlights the robustness needed for the evaluation of Nitrosamines
Enhanced sensitivity for nitrosamine analysis in a metformin active pharmaceutical ingredient (API)
Exceptional quantitative sensitivity of six commonly monitored nitrosamines in losartan
Overcoming N-nitrosamine analysis challenges with mass spectrometry and chromatography solutions
Method developer corner
Routinely achieve low-level quantitation with minimal development time
Routinely achieve low-level quantification with minimal development time
Two approaches for MRM³ data acquisition using clenbuterol
Two approaches for MRM3 data acquisition using the SCIEX 7500 system
Robust bioanalysis over a large 1500 injection sample set
Robust bioanalysis over a large 1500 injection sample set
Achive higher sensitivity to meet the needs of the most challenging assays
Greater sensitivity is achieved through hardware features that enable significant gains in the generation, capture and transmission of ions
Achieve next level sensitivity for the evolution of routine bioanalysis
How the LLOQs for small molecule pharmaceutical compounds in rat plasma are improved using a high-end triple quadrupole mass spectrometer
