cIEF and CZE Charge Heterogeneity Analysis of Biologics
When working with a monoclonal antibody, you need to know the different charge variants to determine identity and stability. You also need the utmost confidence in your results, as the success of your biologic can depend on the accuracy of your results.
Whether you have several methods to develop, samples to QC with limited resources or labs around the world that all need to use the same methods, you need a solution that you can trust. You need to develop charge heterogeneity methods with high resolution and precision that are fast and easy to use, as well as easily transferable across the globe.
The SCIEX PA 800 Plus allows you to confidently assess charge heterogeneity with ultra-high resolution (CIEF) or simplify your sample preparation (CZE). The PA 800 Plus simplifies your workflows and streamlines your methods so you can improve lab productivity and performance. The system also provides you with the raw data required for regulatory acceptance.
Labs that need to analyze mAbs for identity and stability can rapidly obtain high resolution charge heterogeneity information with simple sample preparation, using CZE. You can also develop methods with high resolution and precision, that are fast and easy to use, as well as easily transferable across the globe.
For maximum sensitivity, ultra-high-resolution capillary isoelectric focusing (cIEF) is capable of achieving separation between isoforms as closely related as 0.03 pI units. The SCIEX cIEF workflow on the PA 800 Plus System has proven both robust and portable. Universal or platform methods can also be created – significantly decreasing method development efforts and simplifying workflows with a single method for molecules across a wide pI range.