Explore new frontiers in bioanalysis

Assay efficiency for BBB integrity biomarker quantitation in neurovascular disorder research

The blood-brain barrier (BBB) plays a crucial role in maintaining the central nervous system's stability by preventing harmful substances entry into the brain. Dysfunction of the BBB has been linked to various neurological disorders. To investigate BBB integrity and assess tissue dysfunction or regulation precisely, a sensitive quantitation method is required to analyze extremely low levels of biomarkers in both whole brain tissue and LMD brain samples. 

Here, a reliable and highly sensitive workflow is presented to support the quantitative analysis of [13C12]sucrose using the SCIEX 7500 system coupled with the M5 microflow LC system.

Surfactant protein markers quantified with an antibody-free platform

Sensitive bioanalysis of galactosyl sphingosine (GalSPH) and glucosyl sphingosine (GluSPH) in cerebral spinal fluid

Quantified CSF markers for lysosomal storage disease

Sensitive multiplexed quantitation of protein biomarkers for early drug discovery and development. Low-ng/mL LLOQs were achieved for the 4 surfactant proteins analyzed in human plasma

Sensitive quantitation of an ADC using LC-MRMHR in human serum

TDx was recently approved by FDA (2019) for treating breast, gastric and gastroesophageal where it functions by binding to HER2 of the malignant cells causing targeted DNA damage in cancer cells. It is an anti-HER2 ADC that comprises a HER2 monoclonal AB conjugate via internal cysteine residues.1 TDx has been known to express antitumor activity including in low HER2-expressing cancers. As a result, it is critical to accurately measure levels of ADCs such as TDx in biological matrices for toxicokinetic and pharmacokinetic profiles to meet safety and efficacy requirements.

Immunoaffinity-LC-MRM quantitation of ADCs

Ultra-sensitive LC-MRM analysis for trastuzumab-emtansine quantitation in rat plasma

Quantifying combination mAb therapeutics with multiplexed accurate mass LC-MS/MS

Simultaneous quantitation of trastuzumab and pertuzumab in human serum using accurate mass spectrometry achieved LLOQs of 0.15 μg/mL and strong linearity, with a wide linear dynamic range (LDR) of 3.3 orders of magnitude

High sensitivity MRM workflow for signature peptide quantitation

A new method for increased sensitivity in peptide quantitation is used on a series of surrogate peptides in rat plasma. Ultra-low LLOQs ranging from 5 to 39 fmol/mL are achieved with the SCIEX 7500 system

Sensitive LC-MRMHR approach for cyclic peptide quantitation in human plasma

This technical note demonstrates a reliable and highly sensitive workflow for supporting the quantitative analysis of pasireotide in human plasma using a high-resolution accurate mass spectrometer.

Low-level quantitation of cyclic peptides using the M5 microLC system

Cyclic peptide quantitation using a microflow trap-and-elute method. Low-pg/mL quantitation was achieved for human atrial natriuretic peptide (ANP) in rat plasma

Improved LC-MRM quantitation sensitivity of cyclic peptides (ANP and NP)

Improved LC-MRM quantitation sensitivity for cyclic peptides from the natriuretic peptide family

Low-pg/mL quantitation of cyclic peptides in rat plasma using microflow LC

The quantitation of cyclic peptides using a microflow trap-and-elute method

Sensitive quantitation of the PROTAC degrader ARV-110 in rat plasma

Sensitive analysis of the proteolysis targeting chimera (PROTAC) degrader ARV-110 in rat plasma

High potency drug candidates with improved sensitivity in in-vitro transporter assays

Use of lower compound concentration in transporter assays results in more accurate data that can guide early stage drug development

Low pg/mL quantitation of TL 13-112, a protein targeting chimera (PROTAC) in rat plasma

Highly sensitive, robust, rapid workflow to quantify TL 13-112 and its inactive control, TL 13-110, in rat plasma

A sensitive method for the quantitation of bimatoprost in human plasma

A sensitive method for the quantitation of bimatoprost in human plasma

Next-level sensitivity for the quantitation of warfarin and furosemide in human plasma

Sensitive quantitation of small molecule pharmaceutical compounds, extracted from human plasma, using minimal sample preparation and negative ion mode-based analysis

Sensitive quantitative analysis of semaglutide in rat plasma

Accurate and reproducible MRM-HR quantitative method for PROTAC analysis

Driving more sensitive and selective quantitation of highly potent inhaled corticosteroids

This technical note presents a selective and sensitive approach to quantify the potent inhaled corticosteroids, fluticasone furoate, fluticasone propionate and mometasone furoate, in human plasma using accurate mass spectrometry. This method successfully achieved a lower limit of quantitation (LLOQ) of 1 pg/mL for fluticasone propionate and fluticasone furoate and 2 pg/mL for mometasone furoate in human plasma.

Routinely detect ultra-low levels of two highly potent inhaled compounds

Quantifying fluticasone propionate and salmeterol xinafoate with high sensitivity in human plasma

A sensitive method for the quantitation of mometasone furoate in human plasma

A sensitive method for quantifying a highly potent, orally inhaled drug, mometasone furoate, in human plasma

A sensitive method for the quantitation of formoterol in human plasma

A sensitive method for the quantitation of formoterol, in human plasma, employing a simple liquid-liquid extraction sample preparation

Sensitive quantitation of insulin glargine and its metabolites in human plasma

This technical note presents a reliable and highly sensitive workflow to support the quantitative analysis of insulin glargine and its metabolites M1 and M2 using the SCIEX 7500 system.

Quantitative analysis of IGF-1 and 2 by LC-MRM with scheduled ionization

Quantitation of human insulin-like growth factor-1 and 2 by MRM LC-MS/MS with scheduled ionization

Highly sensitive quantitative workflow for insulin analogs with minimal sample prep

Using intact insulin degludec in rat plasma to demonstrate an LLOQ of 25 pg/mL, with simple protein precipitation sample preparation and an 8-minute LC-MS/MS analysis

Achieve low-pg/mL level quantitation of insulin lispro in rat plasma

Using intact insulin lispro in rat plasma to demonstrate an LLOQ of 50 pg/mL, with simple sample preparation and an 8-minute LC-MS/MS analysis

Ultra-high sensitivity for quantitation of ASO in human plasma

4-fold increase in S/N when quantifying an anti-sense oligonucleotide (ASO) with the new SCIEX 7500 system!

Ultra-low LLOQs for ASOs in rat plasma using a trap-and-elute microflow method

20x improvement in sensitivity for antisense oligonucleotides (ASOs) in rat plasma using a microflow trap-and-elute method

Dos and don'ts of oligonucleotide analysis

Key points to keep in mind when developing your oligonucleotide analysis process

Quantitative performance of a next-generation, highly robust triple quadrupole MS

Bioanalytical laboratories are constantly challenged by the need for reliable triple quadrupole mass spectrometers to ensure the delivery of proper quantitative performance. In order to effectively meet the required drug discovery and development timelines, bioanalytical workflows need robust mass spectrometers to minimize instrument downtime.

Low-ng/mL quantitation of glucagon-like peptide-1 (GLP-1) analog in rat plasma

This technical note demonstrates a sensitive method to quantify a glucagon-like peptide-1 (GLP-1) analog, semaglutide, in rat plasma on a high-end triple quadrupole mass spectrometer. A lower limit of quantitation (LLOQ) of 0.2 ng/mL was determined using a 10-minute LC-MS/MS method.

Superior sensitivity for peptide quantitation in matrix

Quantifying peptides at limits as low as 1 amol, with high accuracy, linearity, and reproducibility

Low pg/mL quantitation of teriparatide in human plasma 

Low-pg/mL quantitation of long-acting insulin analog in rat plasma

Achieving low-pg/mL quantitation of leuprolide in human plasma

Dos and don'ts of oligonucleotide analysis

Key points to keep in mind when developing your oligonucleotide analysis process.

Essential LC-MS method development

5 Habits that can make your LC methods more consistent

Sample preparation made simple