Knowledge Base is your tool to finding answers about common questions. Browse all Knowledge Base articles below.
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Knowledge Base is your tool to finding answers about common questions. Browse all Knowledge Base articles below.
Why do extracted samples and quality controls have lower signal compared to neat standards? When a sample or a spiked quality control is extracted, the signal is usually lower than that of the corresponding standard made in pure solvents.
10/18/2023
Mass Spectrometers
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Standards and Reagents
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Certificates of analysis (CoA) are available for various reagents; however for proteases such as trypsin and beta galactosidase that are sold by SCIEX, no CoA is available.
10/05/2023
Standards and Reagents
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To Prevent Contamination, Use Formic Acid Ampules To prevent your samples from becoming contaminated, use formic acid from small volume ampules rather from a large bottle. Impurities can accrue when taking aliquots of formic acid multiple times from the same bottle, and these impurities can impact positive ion analysis especially.
12/28/2018
Standards and Reagents
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What Are the Peptide Sequences in the Six-Peptide Mixture (PN 4465940)? The six-peptide mixture (part number 4465940) is used for the calibration of mass spectrometers. The kit contains the following six peptides and their mono-isotopic masses: bradykinin (2-9 clip) (904.4681), angiotensin I (1296.6853), glu1-fibrinopeptide (1570.6774), ACTH (1-17 clip) (2093.0867), ACTH (18-39 clip) (2465.1989), and ACTH (7-38 clip) (3657.9294).
12/26/2018
Academia Omics
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Pharma CRO
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Standards and Reagents
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Which Amino Acids in the PepCalMix Are Labeled? The PepCal Mix (P/N 5045759) contains 20 heavy-labeled peptides, with the heavy isotopes incorporated in the amino acids, K and R.
10/09/2018
Academia Omics
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Pharma CRO
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Standards and Reagents
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Where Can I Find Instructions for the Protein Preparation Kit? Protein preparation instructions can be found on the SCIEX website at https://sciex.com/Documents/tech%20notes/Protein-Preparation-Kit-Protocol.pdf or https://sciex.com/products/standards-and-reagents/automated-protein-digestion-solution.
07/09/2018
Academia Omics
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Pharma CRO
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Standards and Reagents
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Where Can I find Safety Data Sheets (MSDS) or Certificates of Analysis (CoA)? To find the Safety Data Sheets and Certificate of Analysis for Sciex reagents, navigate to https://sciex.com/tech-regulatory?safetyTerm=4406127&safetyLanguage=English-United+States
12/11/2017
Standards and Reagents
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Acetic Acid Is Needed To Prepare the PepCalMix Peptides in the SWATH® Acquisition Performance Kit This article describes the experimental issues when formic acid is used to prepare the PepCalMix for the SWATH® Acquisition Performance Kit instead of 5% acetic acid.
11/09/2017
Academia Omics
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Pharma CRO
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Standards and Reagents
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