This video describes a short optimization project to measure the optimal collision energy curves for TripleTOF systems, for performing IDA and SWATH experiments. Rolling collision energy curves were measured on 3 different instruments and then validated on additional instruments. Gains of 5-10% in peptide/protein IDs were found with the new curves. The new curves are compatible with the 100 variable window SWATH method and the Collision Energy Spread (CES) observations are already built into this VW method.
We’d like you to give these new settings a try! You can download the text file or view the table below for the new settings. Enter these values into the IDA CE Pararmeters Table found under the Scripts menu.
The next time you're doing an IDA or SWATH experiment, please try these new collision energy curves and compare them to previous results. Let us know if you see more confident IDs or better quality quantitation. If you have questions on how to do this, please ask!