Lipids are an important part of the metabolome. Their concentration, distribution, and metabolism are affected by genetics and nutrition, as well as various disorders and disease states. However, as a class of molecules, they tend to be difficult to analyze due to their extreme complexity and their highly isobaric nature. In a recent interview with Steve Watkins, Ph.D., Chief Technology Officer of Metabolon, he explains the difficulties of lipid analysis and the challenges associated with creating solutions to accurately characterize the lipidome1. Any solution must be capable of not only identifying the molecular species and the fatty acid composition but also provide accurate quantitation. This provides broad coverage of the lipidome to see a complete picture of the biology - especially in this day and age of Precision Medicine, where the breadth of information is so key.
The SCIEX Lipidyzer™ Platform was designed with these criteria in mind and more and is the first truly integrated complete solution for lipidomics research2. It includes a standardized sample preparation protocol, ready-to-use methods, and automated data processing techniques that enable accurate quantitative lipidomics. The Lipidyzer Platform utilizes a QTRAP® System with SelexION® Technology3 for targeted profiling of over a thousand lipid species from 13 lipid classes for comprehensive coverage of complex lipid metabolism. SelexION Technology is a differential mobility separation (DMS) device that provides the specificity needed for lipid identification. The Lipidomics Workflow Manager (LWM) Software controls the entire workflow. Novel labeled internal standards (over 50 across 13 classes) never seen before in the lipid field were developed specifically for the Lipidyzer Platform, ensure accuracy in identification and quantitation4.
The challenges of understanding complex lipid metabolism and how the Lipidyzer Platform solution meets those challenges are explained well in a recent webinar5. The Lipidyzer Platform maps each lipid molecular species to a matrix of lipid classes and fatty acids and calculates the lipid class concentrations and fatty acid compositions. This streamlined approach making the Lipidyzer Platform is truly a unique turn-key solution allowing the industrialization of quantitative lipidomics.
Lipid analysis can be beneficial to the advancement of so many research areas. And it doesn’t have to be difficult. To learn more about the Lipidyzer platform and lipidomics solutions, read some of our lipid blog articles or view lipid e-learning modules centered around lipid analysis and lipidomics.
- Blog: Why Study Lipids?
- Tech Note: A Novel Lipid Screening Platform that Provides a Complete Solution for Lipidomics Research
- Blog: Resolve the unresolvable. The Power of SelexION® Technology for Lipidomics
- Tech Note: Novel Chemical Standards Kits Enable Facile Lipid Quantitation
- Webinar: Introducing New Solutions for Next-Generation Lipidomics